caa a22 4500 445885475 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0265-6 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0265-6 Stimulation of TLR7 prior to polymicrobial sepsis improves the immune control of the inflammatory response in adult mice [Elektronische Daten] [P. Koerner, T. Traeger, H. Mehmcke, K. Cziupka, W. Kessler, A. Busemann, S. Diedrich, G. Hartmann, C.-D. Heidecke, S. Maier] Objective: The role of Toll-like receptor 7 (TLR7), so far regarded as a receptor for viral RNA, was evaluated in a murine sepsis model. Material: We used the colon ascendens stent peritonitis model (CASP) in female C57B/6 mice. R-848 (1.5μg/g body weight) was injected intravenously prior to sepsis induction. Methods: We determined levels of cytokines by CBA detection kit. Different cell populations were isolated from the spleen by magnetic cell separation and the expression of TLR7 was visualized by immunofluorescence staining. Bacterial load of organs was quantified by incubating suspensions on agar in colony forming units. Results: R-848 application per se led to elevated cytokine levels in serum, spleen and peritoneal cavity. Expression of TLR7 on splenocytes was upregulated following CASP. Bacterial clearance in polymicrobial sepsis was significantly increased in spleen and peritoneum of mice pre-treated with the TLR7-agonist. Cytokine release was regulated in the peritoneum and spleen. Furthermore, apoptosis in thymus and spleen during polymicrobial sepsis was significantly decreased following TLR7 agonist application. Conclusions: TLR7 seems to be essential for pathogen defence not only in viral but also in bacterial infections. Pharmacological stimulation of this receptor prior to induction of sepsis improves the host's capacity to cope with pathogens. Springer Basel AG, 2010 CASP nationallicence Toll like receptor nationallicence R-848 nationallicence Resiquimod nationallicence Sepsis nationallicence Koerner P. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Traeger T. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Mehmcke H. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Cziupka K. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Kessler W. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Busemann A. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Diedrich S. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Hartmann G. Institute of Clinical Pharmacology, Rheinische Friedrich-Wilhelms-Universität, 53105, Bonn, Germany aut Heidecke C.-D Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Maier S. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 271-279 1023-3830 60:3<271 2011 60 11 https://doi.org/10.1007/s00011-010-0265-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0265-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koerner P. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Traeger T. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Mehmcke H. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Cziupka K. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Kessler W. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Busemann A. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Diedrich S. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Hartmann G. Institute of Clinical Pharmacology, Rheinische Friedrich-Wilhelms-Universität, 53105, Bonn, Germany aut NATIONALLICENCE 700 1- Heidecke C.-D Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 700 1- Maier S. Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 271-279 1023-3830 60:3<271 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer