caa a22 4500 445885483 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00011-010-0262-9 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0262-9 Jet exhaust particles alter human dendritic cell maturation [Elektronische Daten] [D. Ferry, C. Rolland, D. Delhaye, F. Barlesi, P. Robert, P. Bongrand, Joana Vitte] Objective and design: Among combustion-derived air pollutants, little is known about jet kerosene characteristics and effects. Materials and methods: Particles yielded by experimental kerosene combustion in a jet engine were characterized with electron microscopy and X-ray energy dispersive spectroscopy. Immature human monocyte-derived dendritic cells were exposed for 18h to 10, 25 or 100μg/mL jet exhaust particles and/or Escherichia coli-derived endotoxin. Antigen-presenting and costimulation molecules (HLA DR, CD40, CD80, CD86, CD11c), tumor necrosis factor-α and interleukin-10 production were measured. Results: The primary particles of jet exhaust are spherical (9.9nm), carbonaceous and exert an adjuvant effect on human monocyte-derived dendritic cell maturation in vitro. Concomitant particle and endotoxin stimulation induced a high cytokine production with low antigen-presenting molecules; particle contact prior to endotoxin contact led to an opposite phenotype. Finally, low cytokine production and high costimulation molecules were present when particle adjunction followed endotoxin contact. Conclusions: Jet exhaust particles act as adjuvants to endotoxin-induced dendritic cell maturation, suggesting possible implications for human health and a role for the time pattern of infectious and pollutant interplay. Springer Basel AG, 2010 Jet exhaust nationallicence Dendritic cell nationallicence Air pollution nationallicence Ambient particulate matter nationallicence Allergy nationallicence Ferry D. Centre Interdisciplinaire de Nanoscience de Marseille CINaM CNRS UPR 3118, Université Aix-Marseille 2, Campus de Luminy, case 913, 13288, Marseille Cedex 9, France aut Rolland C. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut Delhaye D. Atmospheric Environment Unit, ONERA, 29 Avenue de la Division Leclerc, 92320, Chatillon, France aut Barlesi F. Université Aix-Marseille 2, Pôle Cardiovasculaire et Thoracique, Hôpital Nord, Assistance Publique Hôpitaux de Marseille, Service d'Oncologie Multidisciplinaire et Innovations Thérapeutiques, Chemin des Bourrely, 13915, Marseille Cedex 20, France aut Robert P. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut Bongrand P. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut Vitte Joana INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 255-263 1023-3830 60:3<255 2011 60 11 https://doi.org/10.1007/s00011-010-0262-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0262-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferry D. Centre Interdisciplinaire de Nanoscience de Marseille CINaM CNRS UPR 3118, Université Aix-Marseille 2, Campus de Luminy, case 913, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Rolland C. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut NATIONALLICENCE 700 1- Delhaye D. Atmospheric Environment Unit, ONERA, 29 Avenue de la Division Leclerc, 92320, Chatillon, France aut NATIONALLICENCE 700 1- Barlesi F. Université Aix-Marseille 2, Pôle Cardiovasculaire et Thoracique, Hôpital Nord, Assistance Publique Hôpitaux de Marseille, Service d'Oncologie Multidisciplinaire et Innovations Thérapeutiques, Chemin des Bourrely, 13915, Marseille Cedex 20, France aut NATIONALLICENCE 700 1- Robert P. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut NATIONALLICENCE 700 1- Bongrand P. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut NATIONALLICENCE 700 1- Vitte Joana INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/3(2011-03-01), 255-263 1023-3830 60:3<255 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer