caa a22 4500 445885513 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00011-011-0359-9 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0359-9 Paeoniflorin protects against lipopolysaccharide-induced acute lung injury in mice by alleviating inflammatory cell infiltration and microvascular permeability [Elektronische Daten] [Haiqiang Zhou, Difei Bian, Xiaolan Jiao, Zhifeng Wei, Haofang Zhang, Yufeng Xia, Yisheng He, Yue Dai] Objective: The present study aims to explore the effects of paeoniflorin (PF), a monoterpene glycoside isolated from the roots of Paeonia lactiflora Pallas, on acute lung injury (ALI) and the possible mechanisms. Materials and method: ALI was induced in mice by an intratracheal instillation of lipopolysaccharide (LPS, 1mg/kg), and PF was injected intraperitoneally 30min prior to LPS administration. After 24h, lung water content, histology, microvascular permeability and proinflammatory cytokines in the bronchoaveolar lavage fluid were evaluated. Results: It was shown that PF (50, 100mg/kg) could alleviate LPS-induced ALI, evidenced by reduced pulmonary edema, improved histological changes, and attenuated inflammatory cell accumulation in the interstitium and alveolar space as well as microvascular permeability. It also markedly down-regulated the expressions of proinflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF)-α at both transcription and protein levels. Additionally, PF inhibited the phosphorylations of p38 MAP kinase (p38) and c-Jun NH2-terminal kinase (JNK) but not extracellular signal-regulated kinase (ERK), and prevented the activation of nuclear factor-kappa B (NF-κB) in the lung tissues. Conclusion: The findings suggest that PF is able to alleviate ALI, and the underlying mechanisms are probably attributed to decreasing the production of proinflammatory cytokines through down-regulation of the activation of p38, JNK and NF-κB pathways in lung tissues. Springer Basel AG, 2011 Paeoniflorin nationallicence Acute lung injury nationallicence Proinflammatory cytokines nationallicence Permeability nationallicence Signaling pathway nationallicence Zhou Haiqiang Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Bian Difei Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Jiao Xiaolan Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Wei Zhifeng Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Zhang Haofang Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Xia Yufeng Department of Chinese Materia Medica Analysis, China Pharmaceutical University, 24 TongJia Xiang, 210009, Nanjing, China aut He Yisheng Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Dai Yue Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 981-990 1023-3830 60:10<981 2011 60 11 https://doi.org/10.1007/s00011-011-0359-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0359-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Haiqiang Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Bian Difei Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Jiao Xiaolan Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Wei Zhifeng Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Haofang Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Xia Yufeng Department of Chinese Materia Medica Analysis, China Pharmaceutical University, 24 TongJia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- He Yisheng Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Dai Yue Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 981-990 1023-3830 60:10<981 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer