caa a22 4500 445885521 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00011-011-0355-0 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0355-0 Enhanced survival from CLP-induced sepsis following late administration of low doses of anti-IFNγ F(ab′)2 antibody fragments [Elektronische Daten] [R. Márquez-Velasco, A. Martínez-Velázquez, L. Amezcua-Guerra, F. Flores-Guzmán, A. Díaz-Quiñonez, F. Massó, J. Paniagua-Solís, R. Bojalil] Objective: To assess the impact of different doses of anti-interferon gamma (anti-IFNγ) F(ab′)2 fragments, administered prophylactically, on survival and on serum concentration of cytokines in a murine model of sepsis induced by cecal ligation and puncture (CLP). We further explore the impact of therapeutic administration of the most protective dose on survival. Subjects and treatment: Balb/c mice were prophylactically treated by the intraperitoneal route with anti-IFNγ initiated 2h before CLP and every 24h for a total of five times in each of the following doses: 0.01, 0.1, or 1mg/kg. Sham and control groups received sterile saline solution in a similar scheme. Methods: Serum tumor necrosis factor (TNF), interleukin (IL)-1β, IL-6, IL-10 and IFNγ were measured at 3, 24 and 48h after CLP by ELISA. Survival curves were compared using a Mantel-Haenzel method. Results: Significant prophylactic protection was found only with 0.01mg/kg, in association with regulation of IL-1β and IL-10 concentrations. As therapy, anti-IFNγ fragments were protective only when initiated 24h after CLP. Conclusions: Delicate modulation of IFNγ at the correct timing, even when the septic process has begun, is an exciting alternative to explore in the treatment of sepsis. Springer Basel AG, 2011 Sepsis nationallicence Mice nationallicence Anti-interferon gamma nationallicence Modulation nationallicence Cytokine nationallicence Márquez-Velasco R. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut Martínez-Velázquez A. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut Amezcua-Guerra L. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut Flores-Guzmán F. Cancer Unit, German Cancer Research Center, Heidelberg, Germany aut Díaz-Quiñonez A. División de Laboratorios de Vigilancia e Investigación Epidemiológica, CMN La Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico aut Massó F. Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico aut Paniagua-Solís J. Laboratorios Silanes, S. A. de C. V., Amores 1304, 03100, Col del Valle, Mexico City, Mexico aut Bojalil R. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 947-953 1023-3830 60:10<947 2011 60 11 https://doi.org/10.1007/s00011-011-0355-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0355-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Márquez-Velasco R. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut NATIONALLICENCE 700 1- Martínez-Velázquez A. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut NATIONALLICENCE 700 1- Amezcua-Guerra L. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut NATIONALLICENCE 700 1- Flores-Guzmán F. Cancer Unit, German Cancer Research Center, Heidelberg, Germany aut NATIONALLICENCE 700 1- Díaz-Quiñonez A. División de Laboratorios de Vigilancia e Investigación Epidemiológica, CMN La Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico aut NATIONALLICENCE 700 1- Massó F. Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico aut NATIONALLICENCE 700 1- Paniagua-Solís J. Laboratorios Silanes, S. A. de C. V., Amores 1304, 03100, Col del Valle, Mexico City, Mexico aut NATIONALLICENCE 700 1- Bojalil R. Departament of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Tlalpan, 14080, Mexico City, Mexico aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 947-953 1023-3830 60:10<947 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer