caa a22 4500 445885548 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00011-011-0356-z doi (NATIONALLICENCE)springer-10.1007/s00011-011-0356-z Synthetic peptide fragment (65-76) of monocyte chemotactic protein-1 (MCP-1) inhibits MCP-1 binding to heparin and possesses anti-inflammatory activity in stable angina patients after coronary stenting [Elektronische Daten] [T. Arefieva, T. Krasnikova, A. Potekhina, N. Ruleva, P. Nikitin, T. Ksenevich, B. Gorshkov, M. Sidorova, Zh. Bespalova, N. Kukhtina, S. Provatorov, E. Noeva, E. Chazov] Objective and design: The peptide from C-terminal domain of MCP-1 (Ingramon) has been shown to inhibit monocyte migration and possess anti-inflammatory activity in animal models of inflammation and post-angioplasty restenosis. Here, we investigate the effect of Ingramon treatment on blood levels of acute-phase reactants and chemokines in patients after coronary stenting and the mechanisms of Ingramon anti-inflammatory activity. Subjects: Eighty-seven patients with ischemic heart disease (IHD) who faced the necessity of coronary angiography (CA) were enrolled. In 67 patients, one-stage coronary stenting was performed; 33 of them were treated with Ingramon in addition to standard therapy. Twenty patients underwent CA only. Methods: High-sensitivity C-reactive protein (hsCRP) and fibrinogen blood levels were detected routinely. The chemokine concentration in plasma was measured by enzyme-linked immunosorbent assay (ELISA) or cytometric bead array-based immunoassay. Intracellular Ca2+ levels and cell surface integrin exposure were assayed by flow cytometry. MCP-1 dimerization was studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). MCP-1-heparin binding was assessed with a biosensor and ELISA. Results and conclusions: Ingramon treatment was accompanied by less pronounced elevation of hsCRP and fibrinogen levels and decreased MCP-1 concentration in plasma in patients after coronary stenting. Ingramon had no effect on MCP-1 interaction with cell receptors or MCP-1 dimerization, but inhibited MCP-1 binding to heparin. The anti-inflammatory activity of the peptide may be mediated by an impaired chemokine interaction with glycosaminoglycans. Springer Basel AG, 2011 Inflammation nationallicence Chemokines nationallicence MCP-1 nationallicence Glycosaminoglycans nationallicence Peptide inhibitors nationallicence Arefieva T. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Krasnikova T. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Potekhina A. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Ruleva N. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Nikitin P. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut Ksenevich T. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut Gorshkov B. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut Sidorova M. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Bespalova Zh Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Kukhtina N. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Provatorov S. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Noeva E. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Chazov E. Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 955-964 1023-3830 60:10<955 2011 60 11 https://doi.org/10.1007/s00011-011-0356-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0356-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arefieva T. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Krasnikova T. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Potekhina A. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Ruleva N. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Nikitin P. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Ksenevich T. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Gorshkov B. General Physics Institute of Russian Academy of Sciences, Vavilov str., 38, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Sidorova M. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Bespalova Zh Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Kukhtina N. Institute of Experimental Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Provatorov S. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Noeva E. Institute of Clinical Cardiology, Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 700 1- Chazov E. Russian Cardiology Research and Production Complex of Ministry of Health RF, 3rd Cherepkovskaya str., 15, 121552, Moscow, Russia aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 955-964 1023-3830 60:10<955 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer