caa a22 4500 445885602 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00011-011-0349-y doi (NATIONALLICENCE)springer-10.1007/s00011-011-0349-y Modulation of C-reactive protein and tumour necrosis factor-alpha in collagen-induced arthritis in Dark Agouti rats: impact of collagen concentration on severity of arthritis [Elektronische Daten] [Dulanthi Tudave, Ammu Radhakrishnan, Srikumar Chakravarthi, Nagaraja Haleagrahara] Objectives: The study investigated the effect of collagen-induced arthritis in Dark Agouti (DA) rats on the level of C-reactive protein and inflammatory cytokine tumour necrosis factor-alpha (TNF-α). Subjects: Female Dark Agouti (DA) rats. Methods: Three different dosages of (2mg/kg of body weight, 3mg/kg of body weight and 4mg/kg of body weight) collagen and complete Freund's adjuvant suspension were tested. After 45days, serum C-reactive protein, TNF-α, superoxide dismutase and total glutathione assays were done. Radiographic and histopathological changes in the joints were compared. Results: All three groups showed signs of arthritic changes, confirmed by histopathological and radiographic changes. Severe arthritic changes were seen in the rats injected with 4mg/kg of body weight of collagen. There was a significant increase in C-reactive protein, TNF-α, super oxide dismutase and total glutathione levels in the plasma in arthritis rats and the changes were more significant with 4mg/kg of collagen. Conclusion: These results demonstrated that the optimal dose to inject to experimental animals in order to get server arthritic changes was 4mg/kg of collagen with complete Freund's adjuvant suspension. Severe arthritis changes induced significant elevation in plasma C-reactive protein and TNF-α levels. Springer Basel AG, 2011 Arthritis nationallicence Collagen nationallicence C-reactive protein nationallicence Tumour necrosis factor nationallicence Tudave Dulanthi Postgraduate and Research Division, International Medical University, Kuala Lumpur, Malaysia aut Radhakrishnan Ammu Division of Pathology, International Medical University, Kuala Lumpur, Malaysia aut Chakravarthi Srikumar Division of Pathology, International Medical University, Kuala Lumpur, Malaysia aut Haleagrahara Nagaraja Division of Human Biology, International Medical University, Kuala Lumpur, Malaysia aut Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 897-907 1023-3830 60:10<897 2011 60 11 https://doi.org/10.1007/s00011-011-0349-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0349-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tudave Dulanthi Postgraduate and Research Division, International Medical University, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Radhakrishnan Ammu Division of Pathology, International Medical University, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Chakravarthi Srikumar Division of Pathology, International Medical University, Kuala Lumpur, Malaysia aut NATIONALLICENCE 700 1- Haleagrahara Nagaraja Division of Human Biology, International Medical University, Kuala Lumpur, Malaysia aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/10(2011-10-01), 897-907 1023-3830 60:10<897 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer