caa a22 4500 445885661 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0273-6 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0273-6 Liver injury and abscess formation in secondary murine peritonitis [Elektronische Daten] [Andreas Lenz, Mark Fairweather, James Peyton, Sarah Gardner, William Cheadle] Objective: To investigate liver damage and abscess formation in murine, secondary peritonitis. Subjects: Male C57BL/6 mice. Treatment: Intraperitoneal injection with 103CFU Klebsiella pneumoniae and treatment with gentamicin 5mg/kg/day (BID), subcutaneously. Methods: Animals were killed at 12, 24, 48 and 72h after infection. Bacterial burden was determined in the blood and the liver. Liver abscess formation was assessed macroscopically and microscopically. Plasma levels of alkaline phosphatase (ALP) and alanine transaminase (ALT) were measured. Polymorphonuclear leukocyte (PMN) accumulation was assessed via tissue myeloperoxidase (MPO) concentrations. Liver interleukin-10 (IL-10) levels were determined by ELISA. Results: K. pneumoniae was detectable in the blood and the liver at 12h after infection. Liver abscess formation was visible earliest at 24h after infection and most pronounced within the right liver lobes. ALP and ALT levels peaked at 12 and 24h after infection, respectively. MPO was elevated in the right and left liver lobes at 12h but only in the right lobes at 48h after infection, compared to tissue levels in naïve mice. Liver IL-10 concentrations were not significantly increased. Conclusion: Peritonitis led to liver injury and abscess formation but did not significantly affect tissue concentrations of anti-inflammatory IL-10. Springer Basel AG, 2010 Klebsiella pneumoniae nationallicence Liver abscess nationallicence Peritonitis nationallicence Murine nationallicence MPO nationallicence Liver enzymes nationallicence ALP nationallicence ALT nationallicence Lenz Andreas Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut Fairweather Mark Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut Peyton James Veterans Affairs Medical Center-Louisville, 40292, Louisville, Kentucky, USA aut Gardner Sarah Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut Cheadle William Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 337-345 1023-3830 60:4<337 2011 60 11 https://doi.org/10.1007/s00011-010-0273-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0273-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lenz Andreas Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut NATIONALLICENCE 700 1- Fairweather Mark Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut NATIONALLICENCE 700 1- Peyton James Veterans Affairs Medical Center-Louisville, 40292, Louisville, Kentucky, USA aut NATIONALLICENCE 700 1- Gardner Sarah Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut NATIONALLICENCE 700 1- Cheadle William Department of Surgery, University of Louisville, 550 South Jackson Street, 40292, Louisville, Kentucky, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 337-345 1023-3830 60:4<337 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer