caa a22 4500 44588567X CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0280-7 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0280-7 Histopathological features and expression profiles of cytokines, chemokines and SOCS family proteins in trehalose 6,6′-dimycolate-induced granulomatous lesions [Elektronische Daten] [Yusuke Sakai, Kazuyuki Uchida, Hiroyuki Nakayama] Objective and design: The objective of this paper is to elucidate the factors contributing to the development and regression of trehalose 6,6′-dimycolate (TDM)-induced model of tuberculous granulomatous lesions. Materials and treatment: BALB/c mice were twice injected i.p. with a 100μl of w/o/w emulsion (100μg of TDM, 3.2μl of Freund's incomplete adjuvant, 3.2μl of PBS, and 93.6μl of saline containing 0.2% Tween 20) at a 1week interval. The mice were killed at days 0, 3, 7, 14, or 21 after the last injection. Three mice were used per group. Methods: We examined histopathological changes of the lesions and defined the expression levels of cytokines and suppressor of cytokine signaling (SOCS) family proteins by real-time PCR. Results: The levels of inflammatory cytokine, such as TNF-α and IL-1β, paralleled with the size of the lesions and the levels of TGF-β and SOCS-3 were high at regression phase. Discussion: Our results demonstrated that both the down-regulation of inflammatory cytokines and up-regulation of TGF-β and SOCS-3 are crucial for histopathological changes including alteration in the sizes of the lesions and changes in inflammatory cell populations. Springer Basel AG, 2010 Trehalose 6,6′-dimycolate nationallicence Granuloma regression nationallicence Cytokine nationallicence Chemokine nationallicence Suppressor of cytokine signaling family proteins nationallicence Histopathology nationallicence Sakai Yusuke Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Uchida Kazuyuki Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Nakayama Hiroyuki Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 371-378 1023-3830 60:4<371 2011 60 11 https://doi.org/10.1007/s00011-010-0280-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0280-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakai Yusuke Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Uchida Kazuyuki Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakayama Hiroyuki Department of Veterinary Pathology, Graduated School of Agricultural and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 371-378 1023-3830 60:4<371 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer