caa a22 4500 445885696 CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0277-2 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0277-2 The role of nitric oxide during healing of trauma to the skeletal muscle [Elektronische Daten] [Lidiane Filippin, María Cuevas, Elena Lima, Norma Marroni, Javier Gonzalez-Gallego, Ricardo Xavier] Introduction: The role of NO in muscle injury is not clear. Methods: We examined the involvement of the NO system in the development of muscle damage in an experimental model of crush injury. The animals were divided into four groups: (1) control (CO), (2) sham trauma, (3) trauma, (4) trauma+l-NAME, in two experimental phases, 24h and 7days after injury. Results: Twenty-four hours post-trauma, the crushed muscle was characterized by an intense inflammatory reaction. These changes were accompanied by increased oxidative damage, increased cytokine mRNA transcription, NF-κB binding ability and TGF-β growth factor expression in the gastrocnemius muscle. Treatment with l-NAME markedly decreased these histological and molecular abnormalities at 24h. However, at 7days post-trauma, increased collagen formation was observed in the l-NAME group. Discussion: These findings indicate that NO is involved in the balance between fibrosis and healing with regeneration. Springer Basel AG, 2010 Nitric oxide nationallicence Muscle repair nationallicence Oxidative stress nationallicence Nitrative stress nationallicence l -NAME nationallicence Filippin Lidiane Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 90035-003, Porto Alegre, RS, Brazil aut Cuevas María Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut Lima Elena Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut Marroni Norma Experimental Hepatology and Physiology Laboratory of HCPA, Porto Alegre, Brazil aut Gonzalez-Gallego Javier Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut Xavier Ricardo Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 90035-003, Porto Alegre, RS, Brazil aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 347-356 1023-3830 60:4<347 2011 60 11 https://doi.org/10.1007/s00011-010-0277-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0277-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Filippin Lidiane Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 90035-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Cuevas María Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut NATIONALLICENCE 700 1- Lima Elena Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut NATIONALLICENCE 700 1- Marroni Norma Experimental Hepatology and Physiology Laboratory of HCPA, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Gonzalez-Gallego Javier Institute of Biomedicine (IBIOMED), University of Leon, Leon, Spain aut NATIONALLICENCE 700 1- Xavier Ricardo Serviço de Reumatologia, Laboratory of Molecular Biology of Autoimmune and Infectious Disease, Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2350, 90035-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 347-356 1023-3830 60:4<347 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer