caa a22 4500 44588570X CHVBK 20180317145558.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0272-7 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0272-7 Apolipoprotein(a) inhibits lipopolysaccharide-induced IL-6 secretion in human astrocytoma cell line by interfering with lipopolysaccharide signaling [Elektronische Daten] [Guglielmina Chimienti, Anna Mezzapesa, Grazia Liuzzi, Tiziana Latronico, Gabriella Pepe] Objective: To examine the role of lipoprotein(a) [Lp(a)] on the inflammatory response of cells in the nervous system by investigating its effect on lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) secretion. Materials and methods: Human astrocytoma U373 cells were treated with recombinant apolipoprotein(a) [r-apo(a)] A10K (175-11nM), alone or in combination with LPS (100 and 10ng/ml). IL-6 levels were evaluated by immunoblotting. Statistical analysis was performed by one-way ANOVA. Results: r-apo(a) caused dose-dependent inhibition of LPS-induced IL-6 secretion (100ng/ml LPS, p=0.0205; 10ng/ml LPS, p=0.0005). Pre-treatment of cells with 88nM r-apo(a), rinsing, and activation with 10ng/ml LPS did not reverse the inhibition (p=0.0048), which could be reversed by supplementation with excess serum (5-20%) (p=0.0454) or recombinant CD14 (2.0-0.05μg/ml) (p=0.0230). Conclusions: Our data indicate that apo(a) plays a natural anti-endotoxin role which relies on its interference with cell-associated and serum components of LPS signaling. Springer Basel AG, 2010 Apolipoprotein(a) nationallicence Interleukin-6 nationallicence Lipopolysaccharide-induced inflammation nationallicence Lipoprotein(a) nationallicence Recombinant proteins nationallicence Chimienti Guglielmina Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut Mezzapesa Anna Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut Liuzzi Grazia Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut Latronico Tiziana Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut Pepe Gabriella Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 329-335 1023-3830 60:4<329 2011 60 11 https://doi.org/10.1007/s00011-010-0272-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0272-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chimienti Guglielmina Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Mezzapesa Anna Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Liuzzi Grazia Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Latronico Tiziana Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut NATIONALLICENCE 700 1- Pepe Gabriella Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello”, University of Bari, Via Orabona 4, 70126, Bari, Italy aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 329-335 1023-3830 60:4<329 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer