caa a22 4500 445885718 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0279-0 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0279-0 Intermittent high glucose promotes expression of proinflammatory cytokines in monocytes [Elektronische Daten] [Yang Li-bo, Qi Wen-bo, Lv Xiao-hong, Feng You-lun, Zhang Tie] Objective and design: The aim of this study was to examine expression of proinflammatory cytokines in monocytes under fluctuating glucose conditions. Material and treatment: Monocytic cells (THP-1) were divided into four groups and cultured in the presence of 5 or 15mmol/L glucose or in fluctuating conditions (12h exposure to 15mmol/L glucose or mannitol medium followed by 12h exposure to 5mmol/L glucose or mannitol medium) respectively. Methods: Levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in the supernatants and surface expression of CD11b in monocytes were measured after 72h culture. Paired Student's t tests were used to compare two groups and ANOVA for multiple comparisons. Results: Activation of monocytes was most pronounced in the fluctuating glucose conditions, as measured by concentrations of IL-6 and TNF-α in cultured supernatants and surface expression of CD11b in monocytes (P<0.05). Fluctuating mannitol also induced a proinflammatory profile, but to a lesser extent than fluctuating glucose. Conclusions: The results indicated that exposure to fluctuating glucose concentrations enhanced activation of monocytes compared with stable elevation of glucose concentrations. The effects were partly attributable to the inherent osmotic changes. Springer Basel AG, 2010 Intermittent glucose nationallicence Monocyte nationallicence IL-6 nationallicence TNF-α nationallicence CD11b nationallicence Li-bo Yang Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut Wen-bo Qi Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut Xiao-hong Lv Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut You-lun Feng Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut Tie Zhang Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 367-370 1023-3830 60:4<367 2011 60 11 https://doi.org/10.1007/s00011-010-0279-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0279-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li-bo Yang Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut NATIONALLICENCE 700 1- Wen-bo Qi Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut NATIONALLICENCE 700 1- Xiao-hong Lv Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut NATIONALLICENCE 700 1- You-lun Feng Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut NATIONALLICENCE 700 1- Tie Zhang Department of Endocrinology, Taian City Central Hospital, Shandong, 271000,, Taian, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 367-370 1023-3830 60:4<367 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer