caa a22 4500 445885734 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0283-4 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0283-4 Aspirin significantly decreases the nonopsonic phagocytosis and immunogenicity of macrophages in mice [Elektronische Daten] [Aqeel Javeed, Yuzhu Hou, Kaizhong Duan, Baojun Zhang, Hong Shen, Yuhong Cao, Yong Zhao] Objective and design: The effect of acetylsalicyclic acid (ASA) on the phagocytosis and immunogenicity of macrophages remains to be determined. Material or subjects: BALB/c mice and peritoneal macrophages were used. Treatment: BALB/c mice were treated with ASA (0, 6, or 60mg/kg/day) intraperitoneally for 1week. Methods: Flow cytometry and phagocytosis assay were employed. Results: ASA significantly decreased cell numbers, nonopsonic phagocytosis, and immunogenicity as well as changing the phenotypes of peritoneal macrophages in vivo. After in vitro treatment with ASA, macrophages expressed low levels of MHC-II, CD80, and CD47 molecules, and showed significantly decreased phagocytosis. Importantly, ASA blocked the differentiation of macrophages from bone marrow cells in vitro as indicated by decreased macrophage cell numbers and phenotype alteration. Conclusions: The present study provides basic information on the direct effect of ASA on macrophages, supporting the potential application of ASA in patients with allo-grafts or autoimmune diseases. Springer Basel AG, 2010 Aspirin nationallicence Macrophages nationallicence Phagocytosis nationallicence Immunogenicity nationallicence ASA : Aspirin nationallicence BBMs : Bone marrow derived macrophages nationallicence CFSE : Carboxyfluorescein diacetate succinimidyl ester nationallicence CPM : Counts per minute nationallicence cRBCs : Chicken red blood cells nationallicence FCM : Flow cytometry nationallicence FITC : Fluorescein isothiocyanate nationallicence mAbs : Monoclonal antibodies nationallicence MHC : Major histocompatibility complex nationallicence MLR : Mixed leukocyte reaction nationallicence NSAIDs : Nonsteroidal anti-inflammatory drugs nationallicence PE : Phycoerythrin nationallicence PEMs : Peritoneal macrophages nationallicence PGs : Prostaglandins nationallicence PI : Propidium iodide nationallicence TPM : Two-photon microscopy nationallicence Javeed Aqeel Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Hou Yuzhu Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Duan Kaizhong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Zhang Baojun Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Shen Hong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Cao Yuhong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Zhao Yong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 389-398 1023-3830 60:4<389 2011 60 11 https://doi.org/10.1007/s00011-010-0283-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0283-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Javeed Aqeel Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Hou Yuzhu Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Duan Kaizhong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Zhang Baojun Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Shen Hong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Cao Yuhong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 700 1- Zhao Yong Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, 100101, Beijing, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 389-398 1023-3830 60:4<389 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer