caa a22 4500 445885750 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00011-010-0281-6 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0281-6 Expression analysis of the early chemokine response 4h after in vitro traumatic brain injury [Elektronische Daten] [Astrid Fahlenkamp, Mark Coburn, Michael Czaplik, Yu-Mi Ryang, Markus Kipp, Rolf Rossaint, Cordian Beyer] Objective and design: The importance of cytokine- and chemokine-mediated neuroinflammation in the progress of brain injury is becoming increasingly evident. We investigated the early local cytokine and chemokine expression and the development of tissue injury after moderate mechanical hippocampus trauma. Material or subjects: Mouse organotypic hippocampal slice cultures. Treatment: Drop-weight trauma in the CA1 region of the hippocampus. Methods: Staining of necrotic tissue, PCR array and evaluation, real-time PCR, statistical analysis with a two-tailed, independent t test. Results: At 12 and 24h after trauma, the tissue injury spread from the primary mechanical lesion to the entire hippocampal formation. A pronounced up-regulation of distinct chemokine transcripts was found 4h after in vitro traumatic brain injury which preceded the development of the secondary injury. Conclusions: The enhanced expression of inflammatory genes might contribute to the development of the secondary trauma and could pinpoint future neuroinflammatory and neuroprotective targets for research and treatment. Springer Basel AG, 2010 Brain trauma nationallicence Neuroinflammation nationallicence Cytokines nationallicence Chemokines nationallicence Array nationallicence Fahlenkamp Astrid Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut Coburn Mark Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut Czaplik Michael Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut Ryang Yu-Mi Department of Neurosurgery, Klinikum Rechts der Isar, TU Munich, Ismaninger Str. 22, 81675, Munich, Germany aut Kipp Markus Institute for Neuroanatomy, Medical Faculty, RWTH Aachen, Wendlingweg 2, 52074, Aachen, Germany aut Rossaint Rolf Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut Beyer Cordian Institute for Neuroanatomy, Medical Faculty, RWTH Aachen, Wendlingweg 2, 52074, Aachen, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 379-387 1023-3830 60:4<379 2011 60 11 https://doi.org/10.1007/s00011-010-0281-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0281-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fahlenkamp Astrid Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Coburn Mark Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Czaplik Michael Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Ryang Yu-Mi Department of Neurosurgery, Klinikum Rechts der Isar, TU Munich, Ismaninger Str. 22, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Kipp Markus Institute for Neuroanatomy, Medical Faculty, RWTH Aachen, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Rossaint Rolf Department of Anesthesiology, University Hospital Aachen, RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Beyer Cordian Institute for Neuroanatomy, Medical Faculty, RWTH Aachen, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/4(2011-04-01), 379-387 1023-3830 60:4<379 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer