caa a22 4500 445885785 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0328-3 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0328-3 Simplified detection of eosinophils in induced sputum [Elektronische Daten] [Elizabeth Fireman, Brenda Toledano, Nina Buchner, Moshe Stark, Yehuda Schwarz] Objective and design: The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler modification of the process for identifying eosinophilic inflammation from induced sputum (IS) samples. Material: Samples of 81 patients referred for IS assessment of respiratory diseases were studied. Four different assessment approaches were studied in comparison with the conventional method of selecting plugs proposed by the European Respiratory Society/American Thoracic Society task force. Results: One modification of the conventional method of IS processing emerged as simpler to execute and less technologically demanding than the original one (13.0±18.2 vs. 15.4±22.4% eosinophils, P=0.19). Conclusion: The simpler approach should now encourage the use of IS as a convenient procedure for evaluating eosinophilic inflammation in less sophisticated laboratories. Springer Basel AG, 2011 Asthma nationallicence Eosinophilic inflammation nationallicence Induced sputum nationallicence Non-dedicated laboratories nationallicence Fireman Elizabeth Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut Toledano Brenda Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut Buchner Nina Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut Stark Moshe Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut Schwarz Yehuda Department of Pulmonary Diseases, Tel Aviv University, Tel-Aviv, Israel aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 745-750 1023-3830 60:8<745 2011 60 11 https://doi.org/10.1007/s00011-011-0328-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0328-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fireman Elizabeth Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Toledano Brenda Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Buchner Nina Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Stark Moshe Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel aut NATIONALLICENCE 700 1- Schwarz Yehuda Department of Pulmonary Diseases, Tel Aviv University, Tel-Aviv, Israel aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 745-750 1023-3830 60:8<745 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer