caa a22 4500 445885793 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0331-8 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0331-8 Mimic peptides bonding specifically with the first and second extracellular loops of the CC chemokine receptor 5 derived from a phage display peptide library are potent inhibitors of experimental autoimmune encephalomyelitis [Elektronische Daten] [Hui-min Zheng, Yun Jiang, Ju-rong Wang, Xue-lian Gong, Bao-yu Guo] Purpose: To obtain mimic peptides that specifically bind with the first and second extracellular loops (ECL1, ECL2) of the CC chemokine receptor 5 (CCR5) and to study their treatment effects on experimental autoimmune encephalomyelitis (EAE) mice. Methods: A phage display peptide library was applied to screen peptides that bond with ECL1 and ECL2. ELISA and DNA sequence analysis were used to identify positive clones. EAE mice were treated with synthesized peptides by intraperitoneal injection. Results: Eighteen positive clones were obtained and four peptides with sequences STFTTTL, TPIPQLL, SLPLPKP and QTSSAAL were identified. These peptides could significantly protect against and reduce the severity of EAE. The infiltration of monocytes and lymphocytes into the spinal cord decreased significantly in treated mice, while abundant inflammatory cells and demyelination were observed in spinal cords of EAE mice. Conclusion: CCR5 mimic peptides provided a significant protective effect to EAE mice. These potent inhibitory mimic peptides could be useful in the clinical treatment of multiple sclerosis. Springer Basel AG, 2011 CCR5 nationallicence Mimic peptides nationallicence EAE nationallicence Zheng Hui-min Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Jiang Yun Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Wang Ju-rong Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Gong Xue-lian Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Guo Bao-yu Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 759-767 1023-3830 60:8<759 2011 60 11 https://doi.org/10.1007/s00011-011-0331-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0331-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng Hui-min Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Yun Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ju-rong Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Gong Xue-lian Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Guo Bao-yu Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 759-767 1023-3830 60:8<759 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer