caa a22 4500 445885807 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0332-7 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0332-7 Soluble vascular endothelial growth factor (VEGF) receptor-1 inhibits migration of human monocytic THP-1 cells in response to VEGF [Elektronische Daten] [Cansheng Zhu, Zhaojun Xiong, Xiaohong Chen, Zhengqi Lu, Guoyu Zhou, Dunjing Wang, Jian Bao, Xueqiang Hu] Objective: We aimed to investigate the regulation and contribution of vascular endothelial growth factor (VEGF) and sFlt-1(1-3) to human monocytic THP-1 migration. Materials and methods: Ad-sFlt-1/FLAG, a recombinant adenovirus carrying the human sFlt-1(1-3) (the first three extracellular domains of FLT-1, the hVEGF receptor-1) gene, was constructed. L929 cells were infected with Ad-sFlt-1/FLAG and the expression of sFlt-1 was detected by immunofluorescent assay and ELISA. Corning® Transwell® Filter Inserts containing polyethylene terephthalate (PET) membranes with pore sizes of 3μm were used as an experimental model to simulate THP-1 migration. Five VEGF concentrations (0, 0.1, 1, 10 and 100ng/ml), four concentrations of sFlt-1(1-3)/FLAG expression supernatants (0.1, 1, 10 and 100ng/ml), and monocyte chemoattractant protein-1 (MCP-1, 10ng/ml) were used to test the ability of THP-1 cells to migrate through PET membranes. Results: The sFlt-1(1-3) gene was successfully recombined into Ad-sFlt-1/FLAG. sFlt-1(1-3) was expressed in L929 cells transfected with Ad-sFlt-1/FLAG. THP-1 cell migration increased with increasing concentrations of VEGF, while cell migration decreased with increasing concentrations of sFlt1(1-3)/FLAG. sFlt1(1-3)/FLAG had no effect on MCP-1-induced cell migration. Conclusions: This study demonstrated that VEGF is able to elicit a migratory response in THP-1 cells, and that sFlt-1(1-3) is an effective inhibitor of THP-1 migration towards VEGF. Springer Basel AG, 2011 VEGF nationallicence sFlt-1(1-3) gene nationallicence Human monocytic THP-1 nationallicence Migration nationallicence Zhu Cansheng Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Xiong Zhaojun Department of Cardiovascular diseases, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Chen Xiaohong Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Lu Zhengqi Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Zhou Guoyu Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Wang Dunjing Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Bao Jian Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Hu Xueqiang Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 769-774 1023-3830 60:8<769 2011 60 11 https://doi.org/10.1007/s00011-011-0332-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0332-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Cansheng Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xiong Zhaojun Department of Cardiovascular diseases, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiaohong Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lu Zhengqi Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Guoyu Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Dunjing Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Bao Jian Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xueqiang Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 769-774 1023-3830 60:8<769 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer