caa a22 4500 445885815 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0330-9 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0330-9 Barrier protective effects of lycopene in human endothelial cells [Elektronische Daten] [Jae Bae, Jong-Sup Bae] Aim and objective: Lycopene has antioxidant and anticancer effects but its barrier protective effects and underlying mechanism are not fully identified. The potential barrier protective roles of lycopene in lipopolysaccharide (LPS)-activated primary human umbilical vein endothelial cells (HUVECs) were investigated. Methods: We determined the barrier protective effects of lycopene, such as permeability, leukocyte adhesion and migration, and activation of proinflammatory proteins in LPS-activated HUVECs. Results: Lycopene inhibited vascular barrier permeability, expression of cell adhesion molecules, leukocyte adhesion and transendothelial migration in LPS-activated HUVECs. Further studies revealed that lycopene suppressed the production of tumor necrosis factor-alpha (TNF-α) and activation of nuclear factor-κB (NF-κB), CD14 and Toll-like receptor-4 (TLR4). Conclusion: These findings suggest that lycopene has barrier integrity activity, and inhibitory activity on cell adhesion and migration to endothelial cells by blocking the activation of NF-κB, CD14 and TLR4 expression and production of TNF-α, thereby endorsing its usefulness as therapy for vascular inflammatory diseases. Springer Basel AG, 2011 Lycopene nationallicence HUVEC nationallicence Barrier integrity nationallicence Inflammation nationallicence ECM nationallicence HUVECs : Human umbilical vein endothelial cells nationallicence NF-κB : Nuclear factor-κB nationallicence TNF-α : Tumor necrosis factor-alpha nationallicence CAM : Cell adhesion molecule nationallicence VCAM : Vascular cell adhesion molecule nationallicence ICAM : Intracellular cell adhesion molecule nationallicence TEM : Transendothelial migration nationallicence LPS : Lipopolysaccharide nationallicence ECM : Extracellular cell adhesion molecule nationallicence Bae Jae Department of Biological Science, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Republic of Korea aut Bae Jong-Sup Department of Herbal Pharmaceutical Engineering, College of Herbal Bio-Industry, Daegu Haany University, 290 Yugok-dong, 712-715, Gyeongsan, Gyeongsangbuk-do, Republic of Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 751-758 1023-3830 60:8<751 2011 60 11 https://doi.org/10.1007/s00011-011-0330-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0330-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bae Jae Department of Biological Science, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Bae Jong-Sup Department of Herbal Pharmaceutical Engineering, College of Herbal Bio-Industry, Daegu Haany University, 290 Yugok-dong, 712-715, Gyeongsan, Gyeongsangbuk-do, Republic of Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 751-758 1023-3830 60:8<751 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer