caa a22 4500 445885823 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0339-0 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0339-0 The hippocampal fimbria of cuprizone-treated animals as a structure for studying neuroprotection in multiple sclerosis [Elektronische Daten] [M. Kipp, A. Norkus, B. Krauspe, T. Clarner, K. Berger, P. van der Valk, S. Amor, C. Beyer] Objective and design: It has been demonstrated that changes in the normal-appearing white matter (NAWM) in multiple sclerosis precede the appearance of classical lesions. The understanding of NAWM biology in an established disease model might help to clarify why some of them progress to active demyelinating lesions. Material or subjects: C57BL6 male mice (19-21g) were used in this study. Treatment: Demyelination was induced by feeding mice a diet containing 0.2% cuprizone for up to 5weeks. Methods: Routine stainings (luxol fast blue, and hematoxylin and eosin) and immunohistochemistry were performed to assess myelin status and the inflammatory infiltrate. Results: We demonstrated that, in the toxic demyelination cuprizone model, the corpus callosum is severely demyelinated after a 5-week cuprizone challenge (acute demyelination) whereas the fimbria of the hippocampus appear normal in routine myelin stainings. Microgliosis but not astrogliosis is evident after acute demyelination in the fimbria. Interestingly, both regions, the fimbria and the corpus callosum, demonstrated early oligodendrocyte apoptosis as well as intense microglia accumulation and activation. However, only the corpus callosum progresses to actively demyelination lesions whereas the fimbria does not. Conclusions: The applied model appears suitable for elucidating pathways which promote progression of affected tissue to an active lesion. The Author(s), 2011 CNS inflammation nationallicence Inflammatory models nationallicence Kipp M. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut Norkus A. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut Krauspe B. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut Clarner T. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut Berger K. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut van der Valk P. Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands aut Amor S. Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands aut Beyer C. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 723-726 1023-3830 60:8<723 2011 60 11 https://doi.org/10.1007/s00011-011-0339-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0339-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kipp M. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Norkus A. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Krauspe B. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Clarner T. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Berger K. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- van der Valk P. Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Amor S. Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Beyer C. Institute of Neuroanatomy, Faculty of Medicine, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 723-726 1023-3830 60:8<723 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer