caa a22 4500 445885831 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0325-6 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0325-6 PI3K/Akt signaling pathway is involved in the pathogenesis of ulcerative colitis [Elektronische Daten] [Xiao Huang, Jin Xu, Xiao Zhang, Bo Qiu, Lan Peng, Meng Zhang, Hua Gan] Objective: The purpose of this study was to investigate the role of the PI3K/Akt signaling pathway in the pathogenesis of ulcerative colitis (UC). Materials and methods: Mucosal biopsy specimens from 54 active UC cases and adjacent normal tissues from 18 colon cancer cases were investigated. Twenty-eight Balb/c mice were randomly divided into four groups. Dextran sulphate sodium (DSS) solution (5%) was used to develop the mouse colitis model. After treatment with wortmannin (a PI3K inhibitor), disease activity index (DAI) and histological score was determined for each group of mice. Expression of phosphorylated Akt (p-Akt) in UC patients and mouse intestinal mucosa was determined by immunohistochemical staining. We also determined the effect of wortmannin on tumor necrosis factor-α (TNF-α) expression in intestinal biopsy tissues of UC patients and mice with DSS-induced colitis. Results: Wortmannin significantly reduced the level of p-Akt and TNF-α in the colitis tissues of UC patients and DSS-treated mice. Wortmannin significantly alleviated the inflammation of colitis as assessed by DAI and histological score in DSS-treated mice. Conclusion: The PI3K/Akt signal transduction pathway is involved in the regulation and release of pro-inflammatory cytokines such as TNF-α and plays an important role in the development and progression of UC. Springer Basel AG, 2011 Ulcerative colitis nationallicence DSS-induced colitis nationallicence Wortmannin nationallicence PI3K/Akt signal pathway nationallicence TNF-α nationallicence Huang Xiao Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Xu Jin Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Zhang Xiao Medical and Nursing College of Chengdu University, 610031, Chengdu, Sichuan Province, China aut Qiu Bo Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Peng Lan Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Zhang Meng Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Gan Hua Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 727-734 1023-3830 60:8<727 2011 60 11 https://doi.org/10.1007/s00011-011-0325-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0325-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Xiao Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Xu Jin Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Zhang Xiao Medical and Nursing College of Chengdu University, 610031, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Qiu Bo Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Peng Lan Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Zhang Meng Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 700 1- Gan Hua Department of Geriatrics, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 727-734 1023-3830 60:8<727 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer