caa a22 4500 44588584X CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0333-6 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0333-6 Inhibitory effects of lipoteichoic acid from Staphylococcus aureus on platelet function and platelet-monocyte aggregation [Elektronische Daten] [Ben-Quan Wu, Ming-Jun Zhi, Hui Liu, Jing Huang, Yu-Qi Zhou, Tian-Tuo Zhang] Objective: Lipoteichoic acid (LTA) from Staphylococcus aureus has been demonstrated to inhibit agonist-stimulated platelet aggregation. However, its effects on platelet inflammatory mediator release and platelet-monocyte aggregation are still unclear. In the present study, LTA is examined for its anti-inflammatory properties and effects on platelet-monocyte aggregation. Methods: Blood samples were obtained from 5 healthy volunteers who had taken no medicine in the previous 2weeks. Washed platelets were prepared and incubated with LTA (0.5-2.0μg/mL), then platelet aggregation, P-selectin expression, and soluble CD40L (sCD40L) release were measured by light transmission aggregometry, flow cytometry and enzyme-linked immunoassays, respectively. Platelet-monocyte aggregate formation in whole blood was measured by flow cytometry. Thrombin was used as a stimulant. Results: LTA dose-dependently decreased platelet aggregation from 89.32±10.24% to 36.28±9.01% (P<0.05), sCD40L release from 3.28±0.76 to 1.13±0.45ng/mL (P<0.05) and surface P-selectin expression from 82.01±11.20 to 22.78±6.42% (P<0.05). In human whole blood, 1.0μg/mL LTA inhibited platelet-monocyte aggregation from 78.19±10.94 to 38.24+8.74% (P<0.05). Conclusions: These results indicate that LTA from S. aureus can inhibit platelet-dependent inflammatory mediator release and platelet-monocyte aggregation. These findings suggest that LTA-mediated functional alteration of platelets may contribute to immune evasion of S. aureus. Springer Basel AG, 2011 Staphylococcus aureus nationallicence Lipoteichoic acid nationallicence Platelet aggregation nationallicence P-selectin nationallicence Soluble CD40L nationallicence Platelet-monocyte aggregation nationallicence Wu Ben-Quan Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Zhi Ming-Jun Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Liu Hui Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Huang Jing Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Zhou Yu-Qi Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Zhang Tian-Tuo Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 775-782 1023-3830 60:8<775 2011 60 11 https://doi.org/10.1007/s00011-011-0333-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0333-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Ben-Quan Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhi Ming-Jun Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Hui Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Jing Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Yu-Qi Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Tian-Tuo Department of Internal Medicine, Division of Respiratory and Critical Care, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600, Tianhe Street, 510630, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 775-782 1023-3830 60:8<775 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer