caa a22 4500 445885858 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00011-011-0334-5 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0334-5 G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats [Elektronische Daten] [Quan-hui Yang, Da-wei Liu, Xiao-ting Wang, Rong-li Yang, Yan Shi, Yun Long, Hong-zhong Liu, Huai-wu He, Xiang Zhou, Bo Tang] Objective and design: Hepatocytes emerge from a quiescent state into a proliferative state to recover from septic injury. We hypothesize that hepatocyte cell cycle regulation after sepsis potentially contributes to the recovery of liver function. Methods: An animal model of sepsis was induced by cecal ligation and puncture (CLP) in rats. At serial time points after CLP, hepatocyte expression of p21, P53, cyclin D1, cyclin E, CDK2, CDK4 and PCNA was determined by immunoblot analysis, and the DNA content of isolated hepatocytes was analyzed using flow cytometry. Results: Sepsis-induced liver injury of rats was associated with G1 cell cycle arrest. Recovery of liver function was related to cell cycle progression 48h after CLP. The upregulation of p53 and p21 correlated with G1 cell arrest 48h after CLP. The upregulation of cyclin D1/CDK4 and cyclin E/CDK2 also correlated with the G1/S transition 48h after CLP, resulting in PCNA expression. Conclusions: The data suggests that G1 cell cycle arrest and p53, p21, CDKs, cyclins and PCNA expression may be involved in the injury/recovery of liver function after intraperitoneal sepsis. Springer Basel AG, 2011 Liver nationallicence Cyclin nationallicence Cyclin-dependent kinase nationallicence Cyclin-dependent kinase inhibitor nationallicence Yang Quan-hui Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Liu Da-wei Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Wang Xiao-ting Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Yang Rong-li Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Shi Yan Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Long Yun Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Liu Hong-zhong Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut He Huai-wu Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Zhou Xiang Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Tang Bo Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 783-789 1023-3830 60:8<783 2011 60 11 https://doi.org/10.1007/s00011-011-0334-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0334-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Quan-hui Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Liu Da-wei Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Wang Xiao-ting Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Yang Rong-li Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Shi Yan Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Long Yun Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Liu Hong-zhong Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- He Huai-wu Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Zhou Xiang Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 700 1- Tang Bo Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/8(2011-08-01), 783-789 1023-3830 60:8<783 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer