caa a22 4500 445885874 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0287-0 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0287-0 Non-invasive screening of progressive joint defects in the Type II collagen-induced arthritis animal model using radiographic paw images [Elektronische Daten] [Samjin Choi, Su-Jin Chae, Sung Kang, Youjin Cheong, Seung-Jae Hong, Hun-Kuk Park] Objective: This study presents a method for non-invasive screening of progressive arthritic reactions in a representative rheumatoid arthritis (RA) animal model of Type II collagen-induced arthritis (CIA). Materials: Seventeen male DBA/1J mice (9weeks old) were used for the control group (seven) and the CIA group (ten). Treatment: The CIA model was induced by the injection of a Type II collagen and incomplete Freund's adjuvant emulsion. Methods: Arthritic reactions including joint space narrowing (JSN), bone erosion, inflammation, and physiological defects were observed using five non-invasive methods. The degrees of JSN and bone erosion were quantified using macro-radiographs of specific joints and bones, while the severity of inflammation was evaluated by changes in paw volume and visual scoring of edema and erythema. Results: Significant differences were observed in JSN (≤48.29%, p<0.0001), bone erosion (78.30%, p<0.0001), grip strength (p<0.05), and inflammation (91.67% in edema and 194.12% increase in paw volume, p<0.001) between the CIA group and the controls. The relationships between factors exhibited significant positive correlations (R≥0.9229, p<0.05) between radiographic parameters, with symmetries of≥0.9062 (p<0.05) for JSN and≥0.8575 (p<0.001) for bone erosion, 0.8770 (p<0.0001) for inflammatory parameters, and ≥0.9527 (p<0.0001) for radiographic and non-radiographic parameters. Conclusions: Our non-invasive radiographic and non-radiographic methods of documentation were both acceptable for screening joint defects during RA progression. In addition, the results of this study may be useful for monitoring the progression of joint pathology in RA diseases. Springer Basel AG, 2010 Rheumatoid arthritis nationallicence Bone injury nationallicence Joint damage nationallicence Inflammation nationallicence Symmetrical nationallicence Animal models of rheumatoid arthritis nationallicence Choi Samjin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut Chae Su-Jin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut Kang Sung Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut Cheong Youjin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut Hong Seung-Jae Division of Rheumatology, Department of Internal Medicine, College of Medicine, Kyung Hee University Medical Center, 130-702, Seoul, Republic of Korea aut Park Hun-Kuk Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 447-456 1023-3830 60:5<447 2011 60 11 https://doi.org/10.1007/s00011-010-0287-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0287-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Samjin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut NATIONALLICENCE 700 1- Chae Su-Jin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut NATIONALLICENCE 700 1- Kang Sung Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut NATIONALLICENCE 700 1- Cheong Youjin Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut NATIONALLICENCE 700 1- Hong Seung-Jae Division of Rheumatology, Department of Internal Medicine, College of Medicine, Kyung Hee University Medical Center, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park Hun-Kuk Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, Republic of Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 447-456 1023-3830 60:5<447 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer