caa a22 4500 445885912 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0296-z doi (NATIONALLICENCE)springer-10.1007/s00011-010-0296-z Black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expression via blockage of NF-κB and JNK activation in intestinal epithelial cells [Elektronische Daten] [Young-A Song, Young-Lan Park, Sun-Hye Yoon, Kyu-Yeol Kim, Sung-Bum Cho, Wan-Sik Lee, Ik-Joo Chung, Young-Eun Joo] Objective: The aim of this study was to determine the impact of the black tea polyphenol, theaflavin, on the expression of adhesion molecules and activation of lipopolysaccharide (LPS)-induced innate signaling in rat intestinal epithelial (RIE) cells. Methods: The effect of theaflavin on neutrophil adhesion, expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1, LPS-induced nuclear factor-kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) signaling was examined by neutrophil adhesion assay, RT-PCR, Western blotting, immunofluorescence, and electrophoretic mobility shift assay (EMSA). Results: Theaflavin suppressed adhesion of neutrophils to LPS-stimulated RIE cells. LPS-induced ICAM-1 and VCAM-1 expressions were inhibited by theaflavin. LPS-induced IκBα phosphorylation/degradation and nuclear translocation of NF-κB/p65 were blocked by theaflavin. Also, theaflavin blocked NF-κB DNA-binding activity in EMSA. LPS-induced phosphorylation of JNK was inhibited by theaflavin. Bay11-7082 (a NF-κB inhibitor) and SP600125 (a JNK inhibitor) suppressed the LPS-induced ICAM-1 and VCAM-1 mRNA accumulations. Conclusions: These results indicate that black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expressions through blockage of NF-κB and JNK activation in intestinal epithelial cells. Springer Basel AG, 2010 Theaflavin nationallicence Adhesion molecule nationallicence NF-κB nationallicence MAPK nationallicence Intestinal epithelium nationallicence Song Young-A Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Park Young-Lan Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Yoon Sun-Hye Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Kim Kyu-Yeol Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Cho Sung-Bum Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Lee Wan-Sik Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Chung Ik-Joo Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Joo Young-Eun Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 493-500 1023-3830 60:5<493 2011 60 11 https://doi.org/10.1007/s00011-010-0296-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0296-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Young-A Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Park Young-Lan Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Yoon Sun-Hye Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Kim Kyu-Yeol Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Cho Sung-Bum Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Lee Wan-Sik Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Chung Ik-Joo Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 700 1- Joo Young-Eun Department of Internal Medicine, Chonnam National University Medical School, 8 Hak-Dong, 501-757, Dong-ku, Gwangju, Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 493-500 1023-3830 60:5<493 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer