caa a22 4500 445885920 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0297-y doi (NATIONALLICENCE)springer-10.1007/s00011-010-0297-y Diphenyleneiodonium inhibits the activation of mitogen-activated protein kinases and the expression of monocyte chemoattractant protein-1 in Helicobacter pylori -infected gastric epithelial AGS cells [Elektronische Daten] [Soon Cho, Joo Lim, Kyung Kim, Hyeyoung Kim] Objective: To investigate whether NADPH oxidase induces MCP-1 expression and the activation of mitogen-activated protein kinases (MAPKs) in H. pylori-infected gastric epithelial cells. Material: H. pylori in Korean isolates, human gastric epithelial AGS cells Treatment: AGS cells pretreated with or without an NADPH oxidase inhibitor diphenyleneiodonium (DPI) are cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. Methods: Reactive oxygen species (ROS) and MCP-1 were determined by confocal microscopy and enzyme-linked immonosorbent assay. NADPH oxidase activity was measured by lucigenin assay. mRNA expression of MCP-1 was analyzed by reverse transcription-polymerase chain reaction. Levels of MAPKs were assessed by Western blot analysis. Results: H. pylori induced increase in ROS, NADPH oxidase activity, MCP-1 expression, and the activation of MAPKs including extracellular signal-regulated kinases, p38, and jun N-terminal kinases in AGS cells, which was inhibited by DPI. Conclusion: Inhibiting NADPH oxidase by DPI suppresses H. pylori-induced activation of MAPKs and MCP-1 expression in AGS cells. Springer Basel AG, 2010 Helicobacter pylori nationallicence NADPH oxidase nationallicence Monocyte chemoattractant protein-1 nationallicence Mitogen-activated protein kinase nationallicence Gastric epithelial cells nationallicence Cho Soon Department of Pharmacology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 120-752, Seoul, Korea aut Lim Joo Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, 120-749, Seoul, Korea aut Kim Kyung Department of Pharmacology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 120-752, Seoul, Korea aut Kim Hyeyoung Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, 120-749, Seoul, Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 501-507 1023-3830 60:5<501 2011 60 11 https://doi.org/10.1007/s00011-010-0297-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0297-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cho Soon Department of Pharmacology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Lim Joo Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Kyung Department of Pharmacology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 120-752, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Hyeyoung Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, 120-749, Seoul, Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 501-507 1023-3830 60:5<501 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer