caa a22 4500 445885939 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0282-5 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0282-5 Genetic polymorphisms of TP53 and FAS promoter modulate the progression of coronary artery disease after coronary artery bypass grafting: a gender-specific view [Elektronische Daten] [A. Beiras-Fernandez, M. Angele, C. Koutang, P. Lohse, B. Reichart, P. Lohse, S. Eifert] Introduction: Progression of coronary artery disease (CAD) after primary coronary artery bypass grafting (CABG) is frequent and may lead to recurrent symptoms. Various data indicate that apoptosis is the main event occurring during development and progression of atherosclerotic plaque. Plaque vascular smooth muscle cells (VSMCs) are more sensitive than regular VSMCs to TP53-mediated apoptosis. Methods: We investigated EDTA blood of 192 patients (18% female, age 60.9±7.4years) who had primary CABG more than 5years ago. CAD progression was defined as clinical endpoints: re-operation (n=88; 46%), catheter re-intervention (n=58; 30%), or angina at follow-up (n=89; 46%). Apoptotic gene polymorphisms (Toll-like receptor 2 A753G, FAS ligand C-844T, FAS promoter G-670A, TP53 Arg72Pro, and CD14 C-260T) were investigated by PCR-RFLP and compared to healthy controls (n=200, 24% female, age 63.4±5.4). Gender-specific analysis was carried out. Results: Heterozygous, homozygous and wild-type expression of all five genetic polymorphisms showed almost identical distribution between patients with CAD and healthy controls. Looking at clinical endpoints, with GG expression of Toll-like receptor 2 polymorphism and GG expression of FAS promoter polymorphism, results showed a relative increased risk (p=0.09) for recurrent symptoms and re-intervention. Patients with FAS promoter polymorphism with AA expression had an increased risk of suffering from recurrent symptoms (n=28, p=0.04). We found that patients with homozygous expression of TP53 polymorphisms (n=3, all male) were prone to needing re-intervention after prior CABG (p=0.03), but not re-operation. Over a period up to 15years, the re-intervention rate was significantly different in homozygous genotypes of FAS LG, FAS promoter and TP53. Conclusions: Patients presenting with polymorphisms of FAS LG, FAS promoter and TP53 have an increased risk of CAD progression, as they have a higher rate of re-interventions. Springer Basel AG, 2011 Apoptosis nationallicence Atherosclerosis nationallicence Gene expression nationallicence Beiras-Fernandez A. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut Angele M. Department of Surgery, Ludwig Maximilians University Munich, Munich, Germany aut Koutang C. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut Lohse P. Department of Clinical Chemistry, Ludwig Maximilians University Munich, Munich, Germany aut Reichart B. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut Eifert S. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 439-445 1023-3830 60:5<439 2011 60 11 https://doi.org/10.1007/s00011-010-0282-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0282-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beiras-Fernandez A. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Angele M. Department of Surgery, Ludwig Maximilians University Munich, Munich, Germany aut NATIONALLICENCE 700 1- Koutang C. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Lohse P. Department of Clinical Chemistry, Ludwig Maximilians University Munich, Munich, Germany aut NATIONALLICENCE 700 1- Reichart B. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Eifert S. Department of Cardiac Surgery, Ludwig Maximilians University Munich, Marchioninistr. 15, 81366, Munich, Germany aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 439-445 1023-3830 60:5<439 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer