caa a22 4500 445885947 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0289-y doi (NATIONALLICENCE)springer-10.1007/s00011-010-0289-y Cross-linking of CD137 ligand modulates immune responses of thioglycollate-elicited mouse peritoneal macrophages [Elektronische Daten] [Jun-Sang Bae, Hyeong-Sup Kim, Jae Park, Sang-Hyuk Park, Hyeon-Woo Lee] Objective: The aim of this study was to investigate effects of CD137 ligand (CD137L)-mediated reverse signaling on cellular responses in thioglycollate-elicited mouse peritoneal macrophages. Methods: Five-week-old male C57B6 mice were injected intraperitoneally with thioglycollate for 3days to isolate peritoneal macrophages. We counted total cell numbers with a Cell Lab Quanta SC. CD137L expression was examined with a flow cytometer. We also measured expression of inflammatory cytokines by flow cytometry and/or real time-PCR. Results: Cross-linking of CD137L with recombinant CD137-Fc protein (rCD137-Fc) increased total numbers of thioglycollate-elicited mouse peritoneal macrophages (hIgG-Fc- vs. rCD137-Fc-treated group, p<0.05). However, ligation reduced the increase in IL-1β and IL-6 levels. Real-time PCR analysis showed that treatment of cells with rCD137-Fc also reduced transcript levels of IL-1β, IL-6, iNOS and COX2 (hIgG-Fc- vs. rCD137-Fc-treated group, p<0.05), as well as expression of CD137L. Conclusion: Reverse signals initiated by CD137L negatively modulate certain immune functions of thioglycollate-elicited peritoneal macrophages. Springer Basel AG, 2010 CD137L nationallicence Peritoneal macrophages nationallicence TNF nationallicence Reverse signal nationallicence Thioglycollate nationallicence CD137L : CD137 ligand nationallicence TNFR : Tumor necrosis factor receptor nationallicence IL : Interleukin nationallicence PBS : Phosphate buffered saline nationallicence mAb : Monoclonal antibody nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence rCD137-Fc : Recombinant CD137-Fc nationallicence Bae Jun-Sang Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut Kim Hyeong-Sup Department of Prosthodontics, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut Park Jae Department of Pediatric Dentistry, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut Park Sang-Hyuk Department of Conservative Dentistry, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut Lee Hyeon-Woo Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 467-473 1023-3830 60:5<467 2011 60 11 https://doi.org/10.1007/s00011-010-0289-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0289-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bae Jun-Sang Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Hyeong-Sup Department of Prosthodontics, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut NATIONALLICENCE 700 1- Park Jae Department of Pediatric Dentistry, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut NATIONALLICENCE 700 1- Park Sang-Hyuk Department of Conservative Dentistry, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Hyeon-Woo Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 130-701, Seoul, Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 467-473 1023-3830 60:5<467 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer