caa a22 4500 445885963 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00011-010-0294-1 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0294-1 Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells [Elektronische Daten] [Mijung Yeom, Jongbong Park, Bombi Lee, Sang-Yun Choi, Kyoung Kim, Hyejung Lee, Dae-Hyun Hahm] Objective: Lactoferrin (Lf) is known to have anti-cancer and anti-inflammatory activities; however, its therapeutic mechanism has not been defined. In this study, to explain the therapeutic mechanism of Lf, we examined the effect of Lf on endothelial cell activation, leukocyte integration, and angiogenesis in vitro. Methods: Endothelia-leukocyte adhesion assays were used to assess primary cultures of bovine aortic endothelial cells (BAECs) activation following LPS treatment. The mRNA expression of ICAM-1 and proinflammatory cytokines was measured using RT-PCR. Each step of angiogenesis was evaluated in vitro, including endothelial cell proliferation, migration, and tube formation. Proliferation was examined using WST-1 and BrdU incorporation assays, while wound migration assays were used to evaluate cell migration; capillary-like tube formation assays on Matrigel were used to assess tube formation. Results: Lf reduced the adhesion of human monocyte-like THP-1 cells to BAECs by 45%. Lf also reduced mRNA expression of ICAM-1 and proinflammatory cytokines in BAECs. Lf significantly inhibited BAEC proliferation, migration, and tube formation. Conclusions: Lf exerted a potent effect on BAEC activation, suggesting that it might function via an endothelia-based mechanism in the treatment of various diseases, including rheumatoid arthritis and cancer. Springer Basel AG, 2010 Lactoferrin nationallicence Endothelium nationallicence Bovine aortic endothelial cells (BAEC) nationallicence Angiogenesis in vitro nationallicence Yeom Mijung Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut Park Jongbong Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut Lee Bombi Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut Choi Sang-Yun School of Life Science and Biotechnology, Korea University, 136-701, Seoul, Republic of Korea aut Kim Kyoung East-West Bone and Joint Research Institute, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, 134-727, Seoul, Republic of Korea aut Lee Hyejung Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut Hahm Dae-Hyun Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 475-482 1023-3830 60:5<475 2011 60 11 https://doi.org/10.1007/s00011-010-0294-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0294-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeom Mijung Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park Jongbong Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lee Bombi Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Choi Sang-Yun School of Life Science and Biotechnology, Korea University, 136-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Kyoung East-West Bone and Joint Research Institute, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, 134-727, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hyejung Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Hahm Dae-Hyun Department of Oriental Medicine, Acupuncture and Meridian Science Research Center, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, 130-702, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/5(2011-05-01), 475-482 1023-3830 60:5<475 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer