caa a22 4500 445885998 CHVBK 20180317145559.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0249-6 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0249-6 Toll-like receptor 4 can recognize SapC-DOPS to stimulate macrophages to express several cytokines [Elektronische Daten] [Kaihua Lu, Guangfeng Zhao, Hongna Lu, Shuli Zhao, Yuxian Song, Xiaoyang Qi, Yayi Hou] Objective and design: SapC-DOPS is a newly combined compound consisting of saposin C and dioleoylphosphatidylserine (DOPS). Our recent study showed that SapC-DOPS exhibits anti-tumor activity. However, SapC-DOPS has recognition elements of Toll-like receptor (TLR) 2 and TLR4;therefore, we want to know whether SapC-DOPS can induce abnormal immunoreaction via identification TLRs. Methods: We investigated the capacity of SapC-DOPS to induce cytokines in vivo and in vitro and analyzed the involvement of TLR and NF-kB in these cytokines production. Results: SapC-DOPS could activate the cytokine production by peripheral macrophages, enhance the expressions of TLR4 and stimulate the NF-κB nuclear translocation. PDTC, an NF-κB inhibitor, could decrease the SapC-DOPS inducible TNF-α and IL-1β production. Conclusions: SapC-DOPS was similar to LPS in the immune response and may induce the production of cytokines in macrophages via the TLR4 signaling pathway and, at least in part, the alteration of the NF-κB pathway. Springer Basel AG, 2010 SapC-DOPS nationallicence Raw264.7 nationallicence TLRs nationallicence NF-κB nationallicence Lu Kaihua Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Zhao Guangfeng Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Lu Hongna Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Zhao Shuli Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Song Yuxian Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Qi Xiaoyang Jiangsu Changzhou Changji Biotechnology Development Co., Ltd, 213000, Changzhou, People's Republic of China aut Hou Yayi Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 153-161 1023-3830 60:2<153 2011 60 11 https://doi.org/10.1007/s00011-010-0249-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0249-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Kaihua Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Guangfeng Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Lu Hongna Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Shuli Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Song Yuxian Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Qi Xiaoyang Jiangsu Changzhou Changji Biotechnology Development Co., Ltd, 213000, Changzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hou Yayi Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 153-161 1023-3830 60:2<153 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer