caa a22 4500 445886005 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0255-8 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0255-8 Exogenous procalcitonin evokes a pro-inflammatory cytokine response [Elektronische Daten] [Angelike Liappis, Kevin Gibbs, Eric Nylen, Bona Yoon, Richard Snider, Baochong Gao, Kenneth Becker] Objective and design: Procalcitonin (ProCT) is increased in serum of septic patients and those with systemic inflammation. Endogenous levels of ProCT might influence the response of polymorphonuclear leukocytes (PMNs), independently of endotoxin, in clinical disease. Subjects: Healthy human volunteers. Treatment: Recombinant human ProCT (rhProCT). Methods: Whole blood and PMNs were exposed invitro to exogenous rhProCT. Interleukin (IL)-6, IL-8, IL-10, IL-13, tumor necrosis factor-alpha (TNFα), IL-1β, and macrophage inflammatory protein (MIP)-1β (pg/ml) were measured by multiplex suspension bead-array immunoassay, and migration and phagocytosis were measured in PMNs. Results: In a whole-blood model, a dose-dependent increase in IL-6, TNFα, and IL-1β of the cell-free supernatant was noted. Pre-incubation with ProCT, at doses consistent with clinical sepsis, resulted in a decrease in PMN migration without alteration in phagocytosis of Staphylococcus aureus or indirect measurements of bacterial killing. Conclusion: Clinically relevant levels of ProCT influence immunologic responses that may contribute to systemic inflammatory response and septic shock. Springer Basel AG (outside the USA), 2010 Sepsis nationallicence Inflammation nationallicence Procalcitonin nationallicence Leukocyte nationallicence Neutrophil nationallicence Liappis Angelike Medical Service and Section of Infectious Diseases, Veterans Affairs Medical Center, 50 Irving Street, NW 4A155, Washington, DC, USA aut Gibbs Kevin Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut Nylen Eric Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut Yoon Bona Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut Snider Richard Section of Endocrinology, Veterans Affairs Medical Center, 50 Irving Street, NW GE246, 20422, Washington, DC, USA aut Gao Baochong Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut Becker Kenneth Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 203-207 1023-3830 60:2<203 2011 60 11 https://doi.org/10.1007/s00011-010-0255-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0255-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liappis Angelike Medical Service and Section of Infectious Diseases, Veterans Affairs Medical Center, 50 Irving Street, NW 4A155, Washington, DC, USA aut NATIONALLICENCE 700 1- Gibbs Kevin Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut NATIONALLICENCE 700 1- Nylen Eric Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut NATIONALLICENCE 700 1- Yoon Bona Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut NATIONALLICENCE 700 1- Snider Richard Section of Endocrinology, Veterans Affairs Medical Center, 50 Irving Street, NW GE246, 20422, Washington, DC, USA aut NATIONALLICENCE 700 1- Gao Baochong Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut NATIONALLICENCE 700 1- Becker Kenneth Department of Medicine, The George Washington University Medical Center, 2150 Pennsylvania Ave, NW Suite 5411, 20037, Washington, DC, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 203-207 1023-3830 60:2<203 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer