caa a22 4500 445886013 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0253-x doi (NATIONALLICENCE)springer-10.1007/s00011-010-0253-x Effects of HSP70 on the compression force-induced TNF-α and RANKL expression in human periodontal ligament cells [Elektronische Daten] [Masami Mitsuhashi, Masaru Yamaguchi, Tadashi Kojima, Ryo Nakajima, Kazutaka Kasai] Objective and design: The aim of this study was to investigate the effect of heat shock protein 70 (HSP70) on the mRNA expression of tumor necrosis factor-alpha (TNF-α) and receptor activator of nuclear factor-kappa B ligand (RANKL) induced by compressive forces (CF) in human periodontal ligament (hPDL) cells. Material and treatment: hPDL cells were subjected to 1.0, 2.0, or 4.0g/cm2 of CF for 24h, and were treated with recombinant human inducible HSP70 for 12h. Methods: The mRNA expression of HSP27, HSP70, HSP90, TNF-α, RANKL and OPG from hPDL cells subjected to CF was determined by real-time PCR. The protein production of HSP70 was determined by Western blot analysis and ELISA. Results: The mRNA expression of HSP70, TNF-α and RANKL were found to be increased in a time- and magnitude-dependent manner, detectable at 12, 9, and 9h, respectively. TNF-α and RANKL expression gradually decreased at 12h with increasing HSP70 levels, and further decreased thereafter. Furthermore, exogenous HSP70 partially inhibited the CF-induced TNF-α and RANKL expression in a dose-dependent manner at 6 and 12h. Conclusions: These results indicate that HSP70 may modulate the mRNA expression of TNF-α and RANKL in hPDL cells in response to CF. Springer Basel AG, 2010 Cytokines nationallicence Inflammation nationallicence TNF nationallicence Gene expression nationallicence Bone nationallicence Mitsuhashi Masami Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut Yamaguchi Masaru Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut Kojima Tadashi Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut Nakajima Ryo Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut Kasai Kazutaka Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 187-194 1023-3830 60:2<187 2011 60 11 https://doi.org/10.1007/s00011-010-0253-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0253-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mitsuhashi Masami Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masaru Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Kojima Tadashi Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Nakajima Ryo Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Kasai Kazutaka Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, 271-8587, Matsudo, Chiba, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 187-194 1023-3830 60:2<187 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer