caa a22 4500 44588603X CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0242-0 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0242-0 Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells [Elektronische Daten] [C. Huang, Y. Altimova, S. Strange, J. Ebersole] Objective: To investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (DCs), at the mucosal surface. Materials and methods: The cytokine/chemokine profiles of human macrophages and DCs in response to polybacterial challenges were investigated. Results: Oral Gram-negative bacteria elicited significantly greater IL-8 levels from macrophages, compared to Gram-positive bacteria. Gram-positive bacteria did not show synergism in inducing this chemokine from macrophages. In contrast, pairs of oral Gram-negative bacteria elicited synergistic production of IL-8 by macrophages. Similar results were not observed with TNFα, which only appeared additive with the polybacterial challenge. Selected Gram-negative bacterial pairs synergized in IL-6 production by immature DCs. In mature DCs (mDCs), a Porphyromonas gingivalis/Fusobacterium nucleatum and Porphyromonas intermedia/F. nucleatum polybacterial challenge resulted in significant synergism for IL-6 and TNFα levels. However, only the Pi/Fn combination synergized for IL-12 production and there appeared to be no polybacterial effect on IL-10 production by the mDCs. Conclusions: These results indicate that a polybacterial challenge of cells linking innate and adaptive immune responses results in varied response profiles that are dependent upon the characteristics of the microorganisms that are components of the polybacterial complex. Springer Basel AG, 2010 Polymicrobial nationallicence Cytokine nationallicence Macrophage nationallicence Dendritic cells nationallicence Oral bacteria nationallicence Huang C. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut Altimova Y. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut Strange S. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut Ebersole J. Center for Oral Health Research, HSRB 422, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 119-125 1023-3830 60:2<119 2011 60 11 https://doi.org/10.1007/s00011-010-0242-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0242-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang C. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Altimova Y. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Strange S. Center for Oral Health Research, HSRB 161, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut NATIONALLICENCE 700 1- Ebersole J. Center for Oral Health Research, HSRB 422, College of Dentistry, Chandler Medical Center, University of Kentucky, 40536, Lexington, KY, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 119-125 1023-3830 60:2<119 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer