caa a22 4500 445886048 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0250-0 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0250-0 Colocalization of substance P with tumor necrosis factor-α in the lymphocytes and mast cells in gastritis in experimental rats [Elektronische Daten] [Éva Pongor, Károly Altdorfer, Erzsébet Fehér] Objective: Substance P (SP) elicits numerous potent neuroimmunomodulatory effects, increasing the release of tumor necrosis factor alpha (TNF-α). The study aimed to investigate immunoneural communication in experimentally-induced gastritis in rats. Methods: SP-containing nerve fibers and lymphocytes and mast cells were counted in the mucosa of the stomachs of rats using double immunohistochemical and confocal laser microscopic methods, proving colocalization of SP and TNF-α in the lymphocytes and mast cells. Results: In controls, only the nerve fibers showed SP immunoreactivity (IR). However, in gastritis the number of SP-IR fibers and TNF-α IR lymphocytes and mast cells increased significantly (P<0.001); SP-IR fibers were seen in close contact with lymphocytes and mast cells. Numerous lymphocytes (13.1%) and mast cells (10.8%) showed IR for both SP and TNF-α (colocalization) within the same cells. Conclusion: SP release from nerve fibers, lymphocytes and mast cells together with TNF-α can enhance the development of gastric inflammation and participate in tissue damage in gastritis. Springer Basel AG, 2010 Substance P nationallicence Tumor necrosis factor-α nationallicence Gastritis nationallicence Lymphocytes nationallicence Mast cells nationallicence Colocalization nationallicence Pongor Éva Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut Altdorfer Károly Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut Fehér Erzsébet Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 163-168 1023-3830 60:2<163 2011 60 11 https://doi.org/10.1007/s00011-010-0250-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0250-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pongor Éva Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut NATIONALLICENCE 700 1- Altdorfer Károly Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut NATIONALLICENCE 700 1- Fehér Erzsébet Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tüzoltó u. 58, P.O. Box 95, 1450, Budapest, Hungary aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 163-168 1023-3830 60:2<163 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer