caa a22 4500 445886056 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0244-y doi (NATIONALLICENCE)springer-10.1007/s00011-010-0244-y In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors [Elektronische Daten] [M. Jenny, M. Klieber, D. Zaknun, S. Schroecksnadel, K. Kurz, M. Ledochowski, H. Schennach, Dietmar Fuchs] Introduction: Inflammation is crucially involved in a variety of diseases like autoimmune syndromes, cardiovascular and neurodegenerative disorders, cancer, sepsis and allograft rejection. Methods: Freshly isolated human peripheral blood mononuclear cells (PBMCs) are used as a screening assay for anti-inflammatory properties of compounds. Determinations of neopterin production by ELISA and of tryptophan degradation by HPLC are used as read-outs. Results are compared with further markers of immune response and oxidative stress. Results: Phytohaemagglutinin induced significant tryptophan degradation and neopterin formation in PBMC, which correlated with IFN-γ, TNF-α, soluble cytokine receptors and isoprostane-8. Addition of vitamin C and E suppressed the responses dose-dependently. Discussion: The determination of tryptophan degradation and neopterin production in PBMC reflects various pro- and anti-inflammatory cascades that are of relevance also in patients. It constitutes a robust and reliable approach to screen anti-inflammatory or immunosuppressive drugs and may improve throughput, speed and cost-effectiveness in drug discovery. Springer Basel AG, 2010 Tryptophan nationallicence Kynurenine nationallicence Indoleamine 2 nationallicence 3-Dioxygenase nationallicence Neopterin nationallicence Cytokine nationallicence Cytokine receptors nationallicence Interferon-γ nationallicence Jenny M. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut Klieber M. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut Zaknun D. Department of Pediatrics, University Hospital of Vienna, Vienna, Austria aut Schroecksnadel S. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut Kurz K. Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria aut Ledochowski M. Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria aut Schennach H. Institute for Blood Transfusion and Immunology, University Clinics, Innsbruck, Austria aut Fuchs Dietmar Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 127-135 1023-3830 60:2<127 2011 60 11 https://doi.org/10.1007/s00011-010-0244-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0244-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jenny M. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Klieber M. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Zaknun D. Department of Pediatrics, University Hospital of Vienna, Vienna, Austria aut NATIONALLICENCE 700 1- Schroecksnadel S. Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Kurz K. Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria aut NATIONALLICENCE 700 1- Ledochowski M. Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria aut NATIONALLICENCE 700 1- Schennach H. Institute for Blood Transfusion and Immunology, University Clinics, Innsbruck, Austria aut NATIONALLICENCE 700 1- Fuchs Dietmar Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 127-135 1023-3830 60:2<127 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer