caa a22 4500 445886064 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0254-9 doi (NATIONALLICENCE)springer-10.1007/s00011-010-0254-9 E. coli lipopolysaccharide attenuates adenosine A1 receptor-mediated increase in plasma exudation from the hamster cheek pouch [Elektronische Daten] [Xiao-pei Gao, Israel Rubinstein] Objective and design: To determine whether exposure to E. coli lipopolysaccharide (LPS) modulates adenosine A1 receptor-induced increase in plasma exudation from the intact hamster cheek pouch microcirculation. Methods and results: Using intravital microscopy, we found that suffusion of R(−)-N 6-(2-phenylisopropyl)-adenosine (R(−)-PIA) (1.0 and 10.0nM), a selective adenosine A1 receptor agonist, onto the intact cheek pouch elicited significant, concentration-dependent leaky site formation and increase in clearance of fluorescein thioisocyanate-dextran (mol mass, 70kDa) from post-capillary venules (p<0.05). These responses were significantly attenuated by pre-treatment of hamsters with LPS (p<0.05). By contrast, LPS had no significant effects on CGS-21680-, a selective adenosine A2A receptor agonist, bradykinin- and substance P-induced increases in plasma exudation from the cheek pouch. Conclusion: These data indicate that LPS attenuates adenosine A1 receptor-induced increase in plasma exudation in vivo in a specific fashion. We suggest that this phenomenon represents an endogenous anti-inflammatory cue to avoid excessive inflammation during Gram-negative bacterial infections. Springer Basel AG, 2010 Infection nationallicence Inflammation nationallicence Post-capillary venules nationallicence Bradykinin nationallicence Substance P nationallicence Gao Xiao-pei Department of Medicine (M/C 719), College of Medicine, University of Illinois at Chicago, 840 South Wood Street, 60612-4325, Chicago, IL, USA aut Rubinstein Israel Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 195-201 1023-3830 60:2<195 2011 60 11 https://doi.org/10.1007/s00011-010-0254-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0254-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Xiao-pei Department of Medicine (M/C 719), College of Medicine, University of Illinois at Chicago, 840 South Wood Street, 60612-4325, Chicago, IL, USA aut NATIONALLICENCE 700 1- Rubinstein Israel Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 195-201 1023-3830 60:2<195 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer