caa a22 4500 445886072 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0251-z doi (NATIONALLICENCE)springer-10.1007/s00011-010-0251-z β-1,4-Galactosyltransferase-involved in lipopolysaccharide-induced adhesion of schwann cells [Elektronische Daten] [Ling Hu, Huiguang Yang, Jianping Chen, Xiaohong Li, Zhiyun Ben, Xingxin He, Fupeng Zhang, Tao Tao, Chun Cheng, Aiguo Shen] Objective and design: The carbohydrate moieties of glycoprotein are associated with some inflammatory diseases by affecting a wide range of biological functions of cells. This study aimed to investigate the role of β-1,4-galactosyltransferase-I (β-1,4-GalT-I) in adhesion of Schwann cells during inflammation. Subjects: A rat Schwann cell line, RSC 96 was used. Methods: We used western blotting to detect the expression of β-1,4-GalT-I. Flow cytomety was used to measure the galactosylation of glycoproteins on cell surfaces. Immunofluorescent staining was used to examine the expression of α6 integrin, focal adhesion kinase (FAK) and F-actin. Tyrosine phosphorylation of FAK was detected by immunoprecipitation. An adhesion assay was performed to investigate the adhesion of Schwann cells. One-way ANOVA was used to compare differences between the operated and the control group. Results: Schwann cell adhesion was induced by LPS stimulation and was accompanied by upregulation of β-1,4-GalT-I expression and galactosylation of glycoproteins. There was a change of localization of FAK and cytoskeleton organization in LPS treated cells compared with control cells. The pretreated cells enhanced tyrosine phosphorylation of FAK compared with control cells in the adhesion process. With the increased cell surface expression of α6 integrin and β-1,4-GalT-I, the adhesion of Schwann cells on laminin was increased as well. Conclusions: These results suggested that β-1,4-GalT-I may play an important role in adhesion of Schwann cells during inflammation. Springer Basel AG, 2010 Schwann cell nationallicence β-1,4-Galactosyltransferase-I nationallicence Lipopolysaccharide (LPS) nationallicence Glycoprotein nationallicence Integrin nationallicence Hu Ling Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Yang Huiguang Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut Chen Jianping Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Li Xiaohong Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut Ben Zhiyun Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut He Xingxin Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Zhang Fupeng Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Tao Tao Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Cheng Chun Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut Shen Aiguo Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 169-174 1023-3830 60:2<169 2011 60 11 https://doi.org/10.1007/s00011-010-0251-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0251-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Ling Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Huiguang Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jianping Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Li Xiaohong Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ben Zhiyun Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- He Xingxin Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Fupeng Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Tao Tao Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Chun Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Shen Aiguo Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 169-174 1023-3830 60:2<169 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer