caa a22 4500 445886080 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00011-010-0252-y doi (NATIONALLICENCE)springer-10.1007/s00011-010-0252-y Postnatal development of lipopolysaccharide-induced inflammatory response in the brain [Elektronische Daten] [Andres Ortega, Viren Jadeja, Heping Zhou] Objective: This study aimed to characterize postnatal development of lipopolysaccharide (LPS)-induced inflammatory response in the brain. Methods: Postnatal day (P)1, P21 and P70 Sprague-Dawley® rats were treated with saline or 0.25mg/kg LPS for 2h, and the mRNA expression of neuroinflammatory mediators in the brain was determined using reverse transcriptase-polymerase chain reaction (RT-PCR). The kinetics of LPS-induced neuroinflammatory mediators in the brain of P1 and P21 animals was determined using RT-PCR, and the kinetics of LPS-induced cytokines in the serum were determined using ELISA. The basal levels of Toll-like receptor (TLR)-4, CD14, and myeloid differentiation factor 88 (Myd88) were measured at the mRNA and protein levels using RT-PCR and Western blot assay respectively. Results: The mRNA expression levels of cytokines and chemokines were considerably increased in P21 and P70 brains but not significantly altered in P1 brain at 2h following LPS stimulation. Instead, the induction of cytokines and chemokines was significantly delayed in the brain of P1 animals following LPS stimulation, which was associated with diminished Myd88 production in P1 brain. In parallel, the cytokine response in the serum of P1 animals after LPS stimulation was also delayed compared to P21 animals. Conclusions: TLR-4-mediated innate immunity in the brain was significantly delayed in P1 animals, and underwent significant development during the early postnatal period. Springer Basel AG, 2010 Lipopolysaccharide nationallicence Development nationallicence Neuroinflammation nationallicence Brain nationallicence Cytokines nationallicence Ortega Andres Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut Jadeja Viren Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut Zhou Heping Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 175-185 1023-3830 60:2<175 2011 60 11 https://doi.org/10.1007/s00011-010-0252-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-010-0252-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortega Andres Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut NATIONALLICENCE 700 1- Jadeja Viren Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut NATIONALLICENCE 700 1- Zhou Heping Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, 07079, South Orange, NJ, USA aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/2(2011-02-01), 175-185 1023-3830 60:2<175 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer