caa a22 4500 445886129 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00011-011-0362-1 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0362-1 Amino acids exhibit anti-inflammatory effects in human monocytic leukemia cell line, THP-1 cells [Elektronische Daten] [Shunji Hasegawa, Takashi Ichiyama, Ichiro Sonaka, Ayami Ohsaki, Reiji Hirano, Yasuhiro Haneda, Reiji Fukano, Masami Hara, Susumu Furukawa] Objective: The elemental diet is one of the effective therapies for inflammatory bowel disease. However, the mechanism remains unclear, and there have never been reports about the inhibitory effects of amino acids in human monocytes/macrophages. We investigated the inhibitory effects of amino acids on cytokine production or expression of adhesion molecules that are involved in inflammatory diseases, in human monocytes/macrophages. Methods: We examined the inhibitory effects of cysteine, histidine or glycine on the induction of nuclear factor-κB (NF-κB) activation, expression of intracellular adhesion molecule-1 (ICAM-1, CD54) and production of interleukin-8 (IL-8) in THP-1 cells, a human monocytic leukemia cell line, and peripheral blood mononuclear cells (PBMCs) stimulated with tumor necrosis factor-α (TNF-α). Results: Cysteine, histidine and glycine significantly reduced the activation of NF-κB in THP-1 cells stimulated with TNF-α. In addition, cysteine and histidine significantly inhibited the expression of ICAM-1 and production of IL-8 in THP-1 cells and PBMCs. Conclusions: Our results suggest that cysteine and histidine exhibit anti-inflammatory effects in THP-1 cells, and may be responsible for the efficacy of treatment in inflammatory bowel diseases. Springer Basel AG, 2011 Amino acid nationallicence ICAM-1 (CD54) nationallicence IL-8 nationallicence Monocyte/macrophages nationallicence NF-κB nationallicence Hasegawa Shunji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Ichiyama Takashi Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Sonaka Ichiro Marketing Department, Ajinomoto Pharma Co., Ltd., 1-15-1 Kyobashi, Chuo-ku, 104-8315, Tokyo, Japan aut Ohsaki Ayami Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Hirano Reiji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Haneda Yasuhiro Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Fukano Reiji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Hara Masami Faculty of Human Life Sciences, Department of Food and Health Sciences, Jissen Women's University, 4-1-1 Ohsakaue, Hino, 191-8510, Tokyo, Japan aut Furukawa Susumu Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1013-1019 1023-3830 60:11<1013 2011 60 11 https://doi.org/10.1007/s00011-011-0362-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0362-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hasegawa Shunji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Ichiyama Takashi Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Sonaka Ichiro Marketing Department, Ajinomoto Pharma Co., Ltd., 1-15-1 Kyobashi, Chuo-ku, 104-8315, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohsaki Ayami Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Hirano Reiji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Haneda Yasuhiro Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Fukano Reiji Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Hara Masami Faculty of Human Life Sciences, Department of Food and Health Sciences, Jissen Women's University, 4-1-1 Ohsakaue, Hino, 191-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Furukawa Susumu Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, 755-8505, Yamaguchi, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1013-1019 1023-3830 60:11<1013 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer