caa a22 4500 445886153 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00011-011-0364-z doi (NATIONALLICENCE)springer-10.1007/s00011-011-0364-z Interleukin 29 enhances expression of toll receptor 3 and mediates antiviral signals in human keratinocytes [Elektronische Daten] [Sheng-Quan Zhang, Zheng Zhang, Xin Luo, Sen Yang, Yu Chai, Hai-Liang Huang, Xian-Yong Yin, Dao-Jun Hu, Chun-Jun Yang, Jun-Lin Liu, Xue-Jun Zhang] Objective: Interleukin 29 (IL-29) is a class II cytokine and displays numerous immune functions other than its anti-viral and antiproliferation activities. This study is focused on the effect of IL-29 on human keratinocytes (KCs). Methods: Primary KCs were stimulated by various concentrations of IL-29 for different time periods, and antiviral proteins and TLR3 gene expression were then analyzed by real-time PCR. The signal pathways activated by IL-29 in KCs were detected by western blot. The antiviral activity of IL-29 was determined by methylthiazolyldiphenyl-tetrazolium bromide, and small interfering RNA knockdown was used to analyze the role of toll receptor 3 (TLR3) in the antiviral activity of IL-29. Results: IL-29 was able to induce expression of antiviral proteins and TLR3 gene expression in KCs. IL-29 pretreatment strongly enhanced herpes simplex virus type 1 (HSV-1)-induced expression of the interferon β (IFN-β) gene and protected the KCs from HSV-1 challenge. The IL-29 antiviral activity was partially dependent on TLR3 expression induced by this cytokine, and mechanistic studies demonstrated that the regulation of TLR3 expression by IL-29 might be partially dependent on Janus kinase /signal transducer and activator of transcription (JAK-STATs) activation. Conclusion: IL-29-induced TLR3 expression is involved in antiviral activity of IL-29 in KCs, which suggests a feasible method to cure certain viral infections of the skin. Springer Basel AG, 2011 Interleukin 29 nationallicence Human keratinocytes nationallicence Toll receptor 3 nationallicence Antiviral activity nationallicence Zhang Sheng-Quan Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Zhang Zheng Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Luo Xin Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut Yang Sen Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Chai Yu Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut Huang Hai-Liang Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut Yin Xian-Yong Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Hu Dao-Jun Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut Yang Chun-Jun Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Liu Jun-Lin Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Zhang Xue-Jun Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1031-1037 1023-3830 60:11<1031 2011 60 11 https://doi.org/10.1007/s00011-011-0364-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0364-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Sheng-Quan Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Zhang Zheng Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Luo Xin Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Yang Sen Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Chai Yu Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Huang Hai-Liang Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Yin Xian-Yong Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Hu Dao-Jun Department of Biochemistry and Molecular Biology, Anhui Medical University, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Yang Chun-Jun Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Liu Jun-Lin Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 700 1- Zhang Xue-Jun Institute of Dermatology, Department of Dermatology of the 1st Affiliated Hospital, Anhui Medical University, 69 Meishan Road, 230032, Hefei, Anhui, China aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1031-1037 1023-3830 60:11<1031 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer