caa a22 4500 44588617X CHVBK 20180317145600.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00011-011-0367-9 doi (NATIONALLICENCE)springer-10.1007/s00011-011-0367-9 Role of nitric oxide (NO) metabolism and inflammatory mediators in childhood obesity [Elektronische Daten] [András Hrabák, László Derzbach, Ildikó Csuka, Tamás Bajor, Anna Körner] Objective and design: The role of NO and adipocytokines in childhood obesity was studied, supposing that obesity provokes inflammation. Children were admitted to the pediatric clinic for a regular check up because of obesity. Subjects: Obese (n=79) and healthy (n=12) children were selected and divided into subgroups according to their age, gender, glucose tolerance and nitric oxide synthase (NOS II) positivity. Methods: Urine and blood nitrite plus nitrate, the expression of NOS II in white blood cells, serum adipocytokines and clinical characteristics were analyzed in each group. Significance was tested by unpaired two-tailed t test and by ANOVA. Results: NOS II was only detected in the white blood cells of a subgroup (17/79) of obese children. Serum leptin and resistin concentrations were significantly higher, adiponectin was lower compared to healthy children. Significant correlations were observed between serum adiponectin and resistin levels (reciprocal, R 2=0.4), and between body mass index and serum leptin levels. Conclusions: NOS II expression in white blood cells was observed in a minority of patients. Low-grade inflammation in obese children was suggested by the increased resistin levels, particularly in NOS II-positive patients. Correlation between different adipocytokines was restricted for a few subgroups. Springer Basel AG, 2011 Adiponectin nationallicence Leptin nationallicence Resistin nationallicence Nitric oxide nationallicence Obese children nationallicence Akt/PKB : Protein kinase B nationallicence AP-1 : Activator protein 1 nationallicence BMI : Body mass index nationallicence CRP : C-reactive protein nationallicence EDTA : Ethylene-diamine tetraacetate nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence Erk : Extracellular signal-regulated kinase nationallicence HDL : High density lipoprotein nationallicence HEPES : N-(2-hydroxyethyl) piperazinyl-N′-(2-ethane sulfonic acid) nationallicence HOMA : Homeostasis Model Assessment nationallicence IGT : Impaired glucose tolerance nationallicence IFG : Impaired fasting glucose nationallicence IKK : Inhibitor of κB kinase nationallicence IL-6 : Interleukin-6 nationallicence JNK : C-jun terminal kinase nationallicence MAPK : Mitogen-activated protein kinase nationallicence NF-κB : Nuclear factor κB nationallicence NOS : Nitric oxide synthase nationallicence NOx : Nitrite plus nitrate nationallicence PI3K : Phosphatidyl-inositol-3-kinase nationallicence PPARγ : Peroxisome proliferator activated receptor γ nationallicence T1DM : Type 1 diabetes mellitus nationallicence T2DM : Type 2 diabetes mellitus nationallicence TNF-α : Tumor necrosis factor-α nationallicence WBC : White blood cell(s) nationallicence Hrabák András Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, 1444, Budapest, Hungary aut Derzbach László 1st Department of Pediatrics, Semmelweis University, Bókay J. u. 53-54, 1083, Budapest, Hungary aut Csuka Ildikó Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, 1444, Budapest, Hungary aut Bajor Tamás Spinner Hungary Ltd., Selyem u. 2., 7100, Szekszárd, Hungary aut Körner Anna 1st Department of Pediatrics, Semmelweis University, Bókay J. u. 53-54, 1083, Budapest, Hungary aut Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1061-1070 1023-3830 60:11<1061 2011 60 11 https://doi.org/10.1007/s00011-011-0367-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0367-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hrabák András Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, 1444, Budapest, Hungary aut NATIONALLICENCE 700 1- Derzbach László 1st Department of Pediatrics, Semmelweis University, Bókay J. u. 53-54, 1083, Budapest, Hungary aut NATIONALLICENCE 700 1- Csuka Ildikó Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, 1444, Budapest, Hungary aut NATIONALLICENCE 700 1- Bajor Tamás Spinner Hungary Ltd., Selyem u. 2., 7100, Szekszárd, Hungary aut NATIONALLICENCE 700 1- Körner Anna 1st Department of Pediatrics, Semmelweis University, Bókay J. u. 53-54, 1083, Budapest, Hungary aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1061-1070 1023-3830 60:11<1061 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer