caa a22 4500 445886188 CHVBK 20180317145600.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00011-011-0365-y doi (NATIONALLICENCE)springer-10.1007/s00011-011-0365-y Inflammatory effect of advanced glycation end products on human meniscal cells from osteoarthritic knees [Elektronische Daten] [Hideki Hiraiwa, Tadahiro Sakai, Hirohito Mitsuyama, Takashi Hamada, Ryuichiro Yamamoto, Takaaki Omachi, Yohei Ohno, Motoshige Nakashima, Naoki Ishiguro] Objective: To investigate the inflammatory effects of advanced glycation end-products (AGEs) through the receptor for AGE in meniscal cells from osteoarthritic knees, and examine effects of hyaluronan (HA) on AGE-induced inflammation. Methods: Meniscal cells from human osteoarthritic knees were cultured with or without glycolaldehyde-AGE-bovine serum albumin and 800kDa HA. The amount of prostaglandin E2 (PGE2) protein was determined using an enzyme immunoassay system. Expression of cyclooxygenase (COX)-1, COX-2, membrane associated prostaglandin E synthase (mPGES)-1 and cytosolic PGES (cPGES) was analyzed by real-time reverse transcription polymerase chain reaction and western blotting. Results: PGE2 synthesis was significantly increased by AGEs, and AGE-induced PGE2 production was attenuated by addition of HA. While COX-2 and mPGES-1 expression was significantly upregulated by AGEs, COX-1 and cPGES expression was not affected by AGE. AGE-stimulated COX-2 and mPGES-1 expression was attenuated by HA through CD44 (HA receptor). However, the changes in COX-1 and cPGES expression were almost negligible. Conclusion: In meniscal cells from osteoarthritic knees, AGEs increased the production of inflammatory mediators, including PGE2, COX-2 and mPGES-1. Furthermore, HA could decrease AGE-induced production of PGE2, COX-2 and mPGES-1 through CD44. Springer Basel AG, 2011 Advanced glycation end products nationallicence Meniscal cell nationallicence Osteoarthritis nationallicence Inflammatory mediator nationallicence Hyaluronan nationallicence AGEs : Advanced glycation end-products nationallicence BSA : Bovine serum albumin nationallicence RAGE : The receptor for advanced glycation end products nationallicence OA : Osteoarthritis nationallicence HA : Hyaluronan nationallicence PGE2 : Prostaglandin E2 nationallicence COX : Cyclooxygenase nationallicence mPGES : Membrane associated prostaglandin E synthase nationallicence cPGES : Cytosolic prostaglandin E synthase nationallicence Hiraiwa Hideki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Sakai Tadahiro Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Mitsuyama Hirohito Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Hamada Takashi Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Yamamoto Ryuichiro Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Omachi Takaaki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Ohno Yohei Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Nakashima Motoshige Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Ishiguro Naoki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1039-1048 1023-3830 60:11<1039 2011 60 11 https://doi.org/10.1007/s00011-011-0365-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00011-011-0365-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiraiwa Hideki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Sakai Tadahiro Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Mitsuyama Hirohito Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Hamada Takashi Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Yamamoto Ryuichiro Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Omachi Takaaki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Ohno Yohei Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakashima Motoshige Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Ishiguro Naoki Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan aut NATIONALLICENCE 773 0- Inflammation Research SP Birkhäuser Verlag Basel 60/11(2011-11-01), 1039-1048 1023-3830 60:11<1039 2011 60 11 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer