caa a22 4500 445888156 CHVBK 20180317145606.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00535-011-0405-y doi (NATIONALLICENCE)springer-10.1007/s00535-011-0405-y Role of Th-2 cytokines in the development of Barrett's esophagus in rats [Elektronische Daten] [Yukie Kohata, Yasuhiro Fujiwara, Hirohisa Machida, Hirotoshi Okazaki, Hirokazu Yamagami, Tetsuya Tanigawa, Kenji Watanabe, Toshio Watanabe, Kazunari Tominaga, Min Wei, Hideki Wanibuchi, Tetsuo Arakawa] Background: Barrett's esophagus is characterized by a distinct Th-2-predominant cytokine profile, unlike the pro-inflammatory nature of reflux esophagitis. The aim of this study was to examine the role of Th-2 cytokines during the development of Barrett's esophagus, using a rat model. Methods: Barrett's esophagus was induced by Levrat's esophagojejunostomy technique in Brown-Norway (BN) rats. Rats were killed at 8, 15, 30, and 50weeks after the operation. We studied the incidences of esophagitis and Barrett's esophagus, and the mRNA expression of cytokines and CDX2 by real-time reverse transcriptase-polymerase chain reaction and immunohistochemical staining. Finally, we compared the incidence of Barrett's esophagus in BN rats with that in Sprague-Dawley (SD) rats. Results: Esophagitis was found in all rats. Barrett's esophagus appeared 8weeks after the operation, and its incidence and length increased over time. Levels of Th-2 cytokines such as interleukin (IL)-4, IL-10, and IL-13 were significantly increased in Barrett's esophagus as compared to those in non-Barrett's esophagus, while there were no differences in the levels of pro-inflammatory cytokines. The peak of elevated IL-4 mRNA was observed before that of CDX2 mRNA. IL-4 was co-localized in CD4-positive cells and CDX2-positive goblet cells. The incidence of Barrett's esophagus was more common in BN rats (8/10, 80%) than in SD rats (2/7, 28%) at 30weeks. Conclusion: Th-2 cytokines, especially IL-4, may play a crucial role in the development of Barrett's esophagus in an early phase. These results provide understanding of the pathogenesis of Barrett's esophagus from the aspect of the Th-2 immune response. Springer, 2011 Barrett's esophagus nationallicence Th-2 cytokines nationallicence IL-4 nationallicence CDX2 nationallicence CD4 nationallicence Kohata Yukie Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Fujiwara Yasuhiro Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Machida Hirohisa Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Okazaki Hirotoshi Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Yamagami Hirokazu Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Tanigawa Tetsuya Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Watanabe Kenji Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Watanabe Toshio Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Tominaga Kazunari Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Wei Min Department of Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan aut Wanibuchi Hideki Department of Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan aut Arakawa Tetsuo Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut Journal of Gastroenterology Springer Japan 46/7(2011-07-01), 883-893 0944-1174 46:7<883 2011 46 535 https://doi.org/10.1007/s00535-011-0405-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0405-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kohata Yukie Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Fujiwara Yasuhiro Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Machida Hirohisa Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Okazaki Hirotoshi Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Yamagami Hirokazu Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Tanigawa Tetsuya Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Watanabe Kenji Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Watanabe Toshio Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Tominaga Kazunari Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 700 1- Wei Min Department of Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Wanibuchi Hideki Department of Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan aut NATIONALLICENCE 700 1- Arakawa Tetsuo Department of Gastroenterology, Osaka City University Graduate School of Medicine, 1-4-7 Asahimachi, Abenoku, 545-8585, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/7(2011-07-01), 883-893 0944-1174 46:7<883 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer