caa a22 4500 445888180 CHVBK 20180317145606.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00535-011-0456-0 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0456-0 Differential expression of miR-144* as a novel fecal-based diagnostic marker for colorectal cancer [Elektronische Daten] [Murugan Kalimutho, Giovanna Del Vecchio Blanco, Serena Di Cecilia, Pierpaolo Sileri, Micaela Cretella, Francesco Pallone, Giorgio Federici, Sergio Bernardini] Background: MicroRNAs (miRNA) are tiny, noncoding, small, endogenous RNAs that play major roles in neoplastic transformation and could therefore offer a better quantitative and noninvasive method for the diagnosis and prognosis of colorectal cancer (CRC) using feces. In the present study, we screened feces for 648 miRNAs and analyzed the role of miR-144* as a potential CRC diagnostic marker. Methods: Fecal miRNA expression was profiled with RT-pre-amplification-qPCR, and the stability was determined using both endogenous and exogenous miRNA by RT-qPCR. ROC analysis was performed to enhance the diagnosing power of the CRC patients' fecal specimens. Results: We detected 39% of all the miRNAs screened in feces. Endogenous miRNAs are more stable over time and temperature, while exogenous miRNAs degraded rapidly. miR-144* was overexpressed in feces, suggesting that it could be a potent candidate diagnostic marker for CRC detection, with a sensitivity of 74% and a specificity of 87% (n=75, p<0.0001). Moreover, RT-qPCR analysis showed that miR-144* was also overexpressed in paired CRC tissues, thus suggesting its possible utilization as a diagnostic marker. Conclusions: We demonstrated that miRNAs are stable in the fecal microenvironment, and that, among them, miR-144* represents a novel fecal-based diagnostic marker for CRC screening. Nevertheless, our data need to be validated in a large cohort of subjects. Springer, 2011 miR-144* nationallicence Fecal miRNAs nationallicence CRC nationallicence miR-378 nationallicence miRNA : MicroRNA nationallicence fDNA : Fecal DNA nationallicence CRC : Colorectal cancer nationallicence Kalimutho Murugan Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Del Vecchio Blanco Giovanna Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut Di Cecilia Serena Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Sileri Pierpaolo Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut Cretella Micaela Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut Pallone Francesco Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Federici Giorgio Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Bernardini Sergio Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut Journal of Gastroenterology Springer Japan 46/12(2011-12-01), 1391-1402 0944-1174 46:12<1391 2011 46 535 https://doi.org/10.1007/s00535-011-0456-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0456-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kalimutho Murugan Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Del Vecchio Blanco Giovanna Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut NATIONALLICENCE 700 1- Di Cecilia Serena Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Sileri Pierpaolo Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut NATIONALLICENCE 700 1- Cretella Micaela Gastroenterology Unit, University Hospital Tor Vergata, University of Rome Tor Vergata, Rome, Italy aut NATIONALLICENCE 700 1- Pallone Francesco Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Federici Giorgio Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Bernardini Sergio Department of Internal Medicine, University of Rome "Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/12(2011-12-01), 1391-1402 0944-1174 46:12<1391 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer