caa a22 4500 445888261 CHVBK 20180317145606.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00535-011-0460-4 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0460-4 Amelioration of 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice by immunoregulatory dendritic cells [Elektronische Daten] [Shoichi Hoshino, Akiko Kurishima, Muneo Inaba, Yugo Ando, Toshiro Fukui, Kazushige Uchida, Akiyoshi Nishio, Hiroshi Iwai, Takashi Yokoi, Tomoki Ito, Sanae Hasegawa-Ishii, Atsuyoshi Shimada, Ming Li, Kazuichi Okazaki, Susumu Ikehara] Background: Dendritic cells (DCs) are widely distributed throughout the lymphoid and nonlymphoid tissues, and are important initiators of acquired immunity. They also serve as regulators by inducing self-tolerance. However, it has not been thoroughly clarified whether DCs are somehow involved in the regulation or treatment of inflammatory bowel diseases. Methods: We established an ileitis model by transmurally injecting 2,4,6-trinitrobenzene sulfonic acid (TNBS) into the lumen of the ileocolonic junction. The kinetic movement of DCs at the inflammatory sites was analyzed histologically and by flow cytometry, and DCs obtained from the small intestine were analyzed in order to determine the expression of paired immunoglobulin-like receptor-A/B (PIR-A/B) by flow cytometry and quantitative RT-PCR. Furthermore, the regulatory role of DCs was directly determined by a transfer experiment using TNBS-induced colitis model mice. Results: We observed three DC subsets (PIR-A/Bhigh, PIR-A/Bmed, and PIR-A/B− DCs) in the conventional DCs (cDCs) from day 3, and the number of PIR-A/Bmed cDCs increased from the time the inflammatory responses ceased (day 7). PIR-A/Bmed cDCs actually migrated to the inflamed colon, and ameliorated the colitis induced by TNBS when transferred to colitis-induced recipients. The colitis was greatly exacerbated when mice had been treated with the indoleamine-pyrrole 2,3-dioxygenase (IDO) inhibitor 1-methyltryptophan (1-mT) at the time PIR-A/Bmed cDCs were transferred, indicating that the therapeutic ability of PIR-A/Bmed cDCs is partially dependent on IDO. Conclusion: The PIR-A/Bmed cDCs, which increase in number during the final stages of inflammation, can be used to treat colitis via an IDO-dependent mechanism. Springer, 2011 Dendritic cells nationallicence Paired immunoglobulin-like receptors nationallicence TNBS nationallicence Inflammatory bowel disease nationallicence Hoshino Shoichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Kurishima Akiko The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Inaba Muneo First Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan aut Ando Yugo The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Fukui Toshiro The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Uchida Kazushige The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Nishio Akiyoshi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Iwai Hiroshi Department of Otolaryngology, Kansai Medical University, Moriguchi, Osaka, Japan aut Yokoi Takashi First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan aut Ito Tomoki First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan aut Hasegawa-Ishii Sanae Department of Pathology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan aut Shimada Atsuyoshi Department of Pathology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan aut Li Ming Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Okazaki Kazuichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut Ikehara Susumu Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Journal of Gastroenterology Springer Japan 46/12(2011-12-01), 1368-1381 0944-1174 46:12<1368 2011 46 535 https://doi.org/10.1007/s00535-011-0460-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0460-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoshino Shoichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Kurishima Akiko The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Inaba Muneo First Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Ando Yugo The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Fukui Toshiro The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Uchida Kazushige The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Nishio Akiyoshi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Iwai Hiroshi Department of Otolaryngology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Yokoi Takashi First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Ito Tomoki First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Hasegawa-Ishii Sanae Department of Pathology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan aut NATIONALLICENCE 700 1- Shimada Atsuyoshi Department of Pathology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan aut NATIONALLICENCE 700 1- Li Ming Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Okazaki Kazuichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Ikehara Susumu Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/12(2011-12-01), 1368-1381 0944-1174 46:12<1368 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer