caa a22 4500 44588830X CHVBK 20180317145607.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00535-010-0315-4 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0315-4 Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure [Elektronische Daten] [Zhihui Xu, Xiaoqiang Ren, Yan Liu, Xiaodong Li, Siyu Bai, Yanwei Zhong, Lin Wang, Panyong Mao, Huifen Wang, Shaojie Xin, Vincent Wong, Henry Chan, Fabien Zoulim, Dongping Xu] Objective: To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Patients and methods: Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54-1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant. Conclusions: Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome. Springer, 2010 Hepatitis B virus nationallicence Basal core promoter mutation nationallicence Precore mutation nationallicence Genotype nationallicence Acute-on-chronic liver failure nationallicence ACLF : Acute-on-chronic liver failure nationallicence ALF : Acute liver failure nationallicence ALT : Alanine aminotransferase nationallicence BCP : Basal core promoter nationallicence CHB-M : Mild chronic hepatitis B nationallicence CHB-S : Severe chronic hepatitis B nationallicence HBV : Hepatitis B virus nationallicence HCC : Hepatocellular carcinoma nationallicence LC : Liver cirrhosis nationallicence PC : Precore nationallicence PTA : Prothrombin activity nationallicence TBIL : Total bilirubin nationallicence PCR : Polymerase chain reaction nationallicence Xu Zhihui Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Ren Xiaoqiang Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Liu Yan Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Li Xiaodong Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Bai Siyu Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Zhong Yanwei Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Wang Lin Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Mao Panyong Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Wang Huifen Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Xin Shaojie Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Wong Vincent Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China aut Chan Henry Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China aut Zoulim Fabien INSERM, U871, 69003, Lyon, France aut Xu Dongping Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut Journal of Gastroenterology Springer Japan 46/3(2011-03-01), 391-400 0944-1174 46:3<391 2011 46 535 https://doi.org/10.1007/s00535-010-0315-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0315-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Zhihui Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Ren Xiaoqiang Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Liu Yan Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Li Xiaodong Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Bai Siyu Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Zhong Yanwei Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Wang Lin Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Mao Panyong Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Wang Huifen Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Xin Shaojie Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 700 1- Wong Vincent Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Chan Henry Department of Medicine and Therapeutics and Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Zoulim Fabien INSERM, U871, 69003, Lyon, France aut NATIONALLICENCE 700 1- Xu Dongping Viral Hepatitis Research Laboratory, Beijing Institute of Infectious Diseases, 100039, Beijing, China aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/3(2011-03-01), 391-400 0944-1174 46:3<391 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer