caa a22 4500 445888474 CHVBK 20180317145607.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00535-011-0433-7 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0433-7 Expression of angiotensin II type 1 and type 2 receptor mRNAs in the gastric mucosa of Helicobacter pylori -infected Mongolian gerbils [Elektronische Daten] [Mitsushige Sugimoto, Tomoyuki Ohno, Yoshio Yamaoka] Background: The renin-angiotensin system (RAS) plays an important role in normal homeostasis, carcinogenesis-related angiogenesis, and cell proliferation. Helicobacter pylori infection causes infiltration of inflammatory cells into the gastric mucosa and is considered the major cause of gastric cancer. Whether RAS plays a role in H. pylori infection-related gastric diseases remains unclear. We investigated the changes in gastric mucosal angiotensin II type 1 receptor (AT1R) and type 2 receptor (AT2R) mRNA levels throughout the time course of H. pylori infection in Mongolian gerbils. Methods: Mongolian gerbils were infected with wild-type H. pylori (for 12months) or with its isogenic oipA mutant (for 3months). Gastric mucosal AT1R and AT2R mRNA levels were assessed using real-time reverse transcription-polymerase chain reaction. Results: The gastric mucosal AT1R mRNA level was significantly associated with the severity of inflammatory cell infiltration into the gastric mucosa that reached maximal levels at 12months after infection in both the antrum and body. Inflammatory cell infiltration scores and AT1R and AT2R mRNA levels were significantly lower in oipA mutant than wild-type infections. Mucosal AT1R and AT2R mRNA expressions in wild-type H. pylori-infected gerbils with gastric ulcers were significantly higher than in those without ulcers (P<0.01). Conclusions: Gastric mucosal ATR expression gradually increases during the course of H. pylori infection. Up-regulation of the RAS in association with progressive gastric inflammation suggests a potential role of the RAS in gastric carcinogenesis. OipA appears to play a role in AT1R and AT2R expression and the resulting inflammation. Springer, 2011 H. pylori nationallicence Angiotensin II type 1 receptor nationallicence Mongolian gerbil nationallicence OipA nationallicence Renin-angiotensin system nationallicence ACE : Angiotensin-I converting enzyme nationallicence ARB : AT1R blocker nationallicence ATR : Angiotensin II receptor nationallicence AT1R : Angiotensin II type 1 receptor nationallicence AT2R : Angiotensin II type 2 receptor nationallicence CMA : Chymase nationallicence COX : Cyclooxygenase nationallicence FBS : Fetal bovine serum nationallicence MNC : Mononuclear cell nationallicence OipA : Outer inflammatory protein nationallicence PMN : Polymorphonuclear cell nationallicence RAS : Renin-angiotensin system nationallicence RT-PCR : Real-time reverse transcription-polymerase chain reaction nationallicence Sugimoto Mitsushige Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut Ohno Tomoyuki Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut Yamaoka Yoshio Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut Journal of Gastroenterology Springer Japan 46/10(2011-10-01), 1177-1186 0944-1174 46:10<1177 2011 46 535 https://doi.org/10.1007/s00535-011-0433-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0433-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugimoto Mitsushige Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut NATIONALLICENCE 700 1- Ohno Tomoyuki Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut NATIONALLICENCE 700 1- Yamaoka Yoshio Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/10(2011-10-01), 1177-1186 0944-1174 46:10<1177 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer