caa a22 4500 445888547 CHVBK 20180317145608.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00535-011-0439-1 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0439-1 Correlation between endocytoscopy and conventional histopathology in microstructural features of ulcerative colitis [Elektronische Daten] [Rieko Bessho, Takanori Kanai, Naoki Hosoe, Taku Kobayashi, Tetsuro Takayama, Nagamu Inoue, Makio Mukai, Haruhiko Ogata, Toshifumi Hibi] Background: Routine diagnosis of the histopathological activity of ulcerative colitis (UC) requires multiple biopsy samples, and an endocytoscopy system (ECS) provides real-time ultra-magnifying microscopic imaging in vivo. Methods: We have established an ECS score (ECSS) to determine a histopathological activity index of UC. Fifty-five UC patients (mean age 40.7years; 67% men) were enrolled. A super-magnifying ECS with magnification 450× was used, and sample biopsies were obtained. Matts' histopathological grade was determined, to evaluate disease severity, by two pathologists, with consensus. The ECSS of UC was independently determined by at least two investigators, with consensus. In total, 76 pairs of ECSS and Matts' histopathological grades were independently acquired. To validate the ECSS, inter-observer agreement between three endoscopists, with consensus, and another endoscopist, was calculated as the kappa value. We also evaluated the correlation between the ECSS and Matts' histopathological grade, and between the conventional Matts' endoscopic grade and Matts' histopathological grade. Results: The ECSS of UC intestinal mucosa, i.e., the sum of the indices for shape (0-3) and distance between crypts (0-2), and the visibility of superficial microvessels (0-1), showed a strong correlation with Matts' histopathological grades (ρ=0.713, P<0.001); as well, there was a strong correlation between the conventional Matts' endoscopic grade and Matts' histopathological grade (ρ=0.694, P<0.001). Furthermore, the ECSS showed high reproducibility (κ=0.79, 95% confidence interval [CI] 0.71-0.87). Conclusions: Our novel ECSS has good predictive value for the histopathological activity of UC. Springer, 2011 Endocytoscopy nationallicence Ulcerative colitis nationallicence Mucosal inflammation nationallicence Histopathology nationallicence Bessho Rieko Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut Kanai Takanori Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut Hosoe Naoki Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut Kobayashi Taku Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut Takayama Tetsuro Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut Inoue Nagamu Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut Mukai Makio Division of Diagnostic Pathology, School of Medicine, Keio University, Tokyo, Japan aut Ogata Haruhiko Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut Hibi Toshifumi Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut Journal of Gastroenterology Springer Japan 46/10(2011-10-01), 1197-1202 0944-1174 46:10<1197 2011 46 535 https://doi.org/10.1007/s00535-011-0439-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0439-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bessho Rieko Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut NATIONALLICENCE 700 1- Kanai Takanori Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut NATIONALLICENCE 700 1- Hosoe Naoki Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut NATIONALLICENCE 700 1- Kobayashi Taku Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut NATIONALLICENCE 700 1- Takayama Tetsuro Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut NATIONALLICENCE 700 1- Inoue Nagamu Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut NATIONALLICENCE 700 1- Mukai Makio Division of Diagnostic Pathology, School of Medicine, Keio University, Tokyo, Japan aut NATIONALLICENCE 700 1- Ogata Haruhiko Center for Diagnostic and Therapeutic Endoscopy, School of Medicine, Keio University, Tokyo, Japan aut NATIONALLICENCE 700 1- Hibi Toshifumi Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 160-8582, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/10(2011-10-01), 1197-1202 0944-1174 46:10<1197 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer