caa a22 4500 445888725 CHVBK 20180317145608.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00535-010-0368-4 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0368-4 Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis [Elektronische Daten] [Akira Andoh, Hirotsugu Imaeda, Tomoki Aomatsu, Osamu Inatomi, Shigeki Bamba, Masaya Sasaki, Yasuharu Saito, Tomoyuki Tsujikawa, Yoshihide Fujiyama] Background: Terminal restriction fragment length polymorphism (T-RFLP) analysis is a powerful tool to assess the diversity of a microbial community. In this study, we performed T-RFLP analysis of the fecal microbiota from patients with ulcerative colitis (UC) and those with Crohn's disease (CD). Methods: Thirty-one patients with UC, 31 patients with CD, and 30 healthy individuals were enrolled. The polymerase chain reaction (PCR) products obtained from the 16S rRNA genes of fecal samples were digested with BslI, and T-RF lengths were determined. Results: The fecal microbial communities were classified into 5 clusters. Twenty-eight of the 30 healthy individuals and 17 of the 18 patients with inactive UC were classified into clusters I, II, and III, but these clusters included a small number of patients with active UC and inactive/active CD. In contrast, 8 of the 13 patients with active UC and the majority of CD patients (12 of the 16 patients with inactive CD, and 11 of the 15 patients with active CD) were included in clusters IV and V. Based on the BslI-digested T-RFLP database, the bacteria showed a significant decrease in the Clostridium family in patients with active UC and inactive/active CD. In contrast, Bacteroides were significantly increased in CD patients. No significant differences were observed between patients with active UC and those with active CD. Conclusion: The fecal microbial communities of IBD patients were different from those of healthy individuals. The gut microbiota of patients with inactive UC tended to be closer to that of healthy individuals, suggesting different roles for the fecal microbiota in the pathophysiology of UC and CD. Springer, 2011 Inflammatory bowel disease nationallicence T-RFLP nationallicence Clostridium nationallicence Bacteroides nationallicence Andoh Akira Division of Mucosal Immunology, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Imaeda Hirotsugu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Aomatsu Tomoki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Inatomi Osamu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Bamba Shigeki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Sasaki Masaya Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Saito Yasuharu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Tsujikawa Tomoyuki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Fujiyama Yoshihide Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 479-486 0944-1174 46:4<479 2011 46 535 https://doi.org/10.1007/s00535-010-0368-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0368-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Andoh Akira Division of Mucosal Immunology, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Imaeda Hirotsugu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Aomatsu Tomoki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Inatomi Osamu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Bamba Shigeki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Sasaki Masaya Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Saito Yasuharu Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Tsujikawa Tomoyuki Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 700 1- Fujiyama Yoshihide Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, 520-2192, Otsu, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 479-486 0944-1174 46:4<479 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer