caa a22 4500 44588875X CHVBK 20180317145608.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00535-011-0373-2 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0373-2 Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen: a multicenter, observer-blinded validation study [Elektronische Daten] [Tatsuo Kanda, Akihiro Tsukahara, Kyo Ueki, Yasuo Sakai, Tatsuo Tani, Atsushi Nishimura, Toshiyuki Yamazaki, Yoichi Tamiya, Tetsuya Tada, Masaki Hirota, Jun Hasegawa, Hiroyuki Funaoka, Hiroshi Fujii, Katsuyoshi Hatakeyama] Background: Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Methods: Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Results: Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7±117.9ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8±15.6ng/ml) and those with non-small bowel disease (1.8±1.7ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Conclusion: Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia. Springer, 2011 Acute abdomen nationallicence Biomarker nationallicence ELISA nationallicence I-FABP nationallicence Intestinal ischemia nationallicence Kanda Tatsuo Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut Tsukahara Akihiro Department of Surgery, Niigata Prefecture Shibata Hospital, Shibata, Japan aut Ueki Kyo Department of Surgery, Kariwagun General Hospital, Kashiwazaki, Japan aut Sakai Yasuo Department of Surgery, Saiseikai Niigata Daini Hospital, Niigata, Japan aut Tani Tatsuo Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut Nishimura Atsushi Department of Surgery, Nagaoka Chuo General Hospital, Nagaoka, Japan aut Yamazaki Toshiyuki Department of Surgery, Niigata City General Hospital, Niigata, Japan aut Tamiya Yoichi Department of Surgery, Niigata Prefecture Yoshida Hospital, Tsubame, Japan aut Tada Tetsuya Department of Surgery, Tachikawa General Hospital, Nagaoka, Japan aut Hirota Masaki Department of Surgery, Niigata Prefecture Muikamachi Hospital, Minamiuonuma, Japan aut Hasegawa Jun Department of Surgery, Japanese Red Cross Nagaoka Hospital, Nagaoka, Japan aut Funaoka Hiroyuki DS Pharma Biomedical Co., Ltd., Osaka, Japan aut Fujii Hiroshi Department of Bioscience and Biotechnology, Shinshu University, Nagano, Japan aut Hatakeyama Katsuyoshi Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 492-500 0944-1174 46:4<492 2011 46 535 https://doi.org/10.1007/s00535-011-0373-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0373-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanda Tatsuo Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut NATIONALLICENCE 700 1- Tsukahara Akihiro Department of Surgery, Niigata Prefecture Shibata Hospital, Shibata, Japan aut NATIONALLICENCE 700 1- Ueki Kyo Department of Surgery, Kariwagun General Hospital, Kashiwazaki, Japan aut NATIONALLICENCE 700 1- Sakai Yasuo Department of Surgery, Saiseikai Niigata Daini Hospital, Niigata, Japan aut NATIONALLICENCE 700 1- Tani Tatsuo Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut NATIONALLICENCE 700 1- Nishimura Atsushi Department of Surgery, Nagaoka Chuo General Hospital, Nagaoka, Japan aut NATIONALLICENCE 700 1- Yamazaki Toshiyuki Department of Surgery, Niigata City General Hospital, Niigata, Japan aut NATIONALLICENCE 700 1- Tamiya Yoichi Department of Surgery, Niigata Prefecture Yoshida Hospital, Tsubame, Japan aut NATIONALLICENCE 700 1- Tada Tetsuya Department of Surgery, Tachikawa General Hospital, Nagaoka, Japan aut NATIONALLICENCE 700 1- Hirota Masaki Department of Surgery, Niigata Prefecture Muikamachi Hospital, Minamiuonuma, Japan aut NATIONALLICENCE 700 1- Hasegawa Jun Department of Surgery, Japanese Red Cross Nagaoka Hospital, Nagaoka, Japan aut NATIONALLICENCE 700 1- Funaoka Hiroyuki DS Pharma Biomedical Co., Ltd., Osaka, Japan aut NATIONALLICENCE 700 1- Fujii Hiroshi Department of Bioscience and Biotechnology, Shinshu University, Nagano, Japan aut NATIONALLICENCE 700 1- Hatakeyama Katsuyoshi Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, 951-8510, Niigata, Niigata, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 492-500 0944-1174 46:4<492 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer