caa a22 4500 445888776 CHVBK 20180317145608.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00535-010-0364-8 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0364-8 The specific linker phosphorylation of Smad2/3 indicates epithelial stem cells in stomach; particularly increasing in mucosae of Helicobacter -associated gastritis [Elektronische Daten] [Toshiro Fukui, Masanobu Kishimoto, Atsushi Nakajima, Masao Yamashina, Shinji Nakayama, Takeo Kusuda, Yutaku Sakaguchi, Katsunori Yoshida, Kazushige Uchida, Akiyoshi Nishio, Koichi Matsuzaki, Kazuichi Okazaki] Background: The gastric corpus and antrum are believed to contain epithelial stem cells in the isthmus. However, the lack of useful markers has hindered studies of their origin. We explored whether Smad2/3, phosphorylated at specific linker threonine residues (pSmad2/3L-Thr), could serve as a marker for stem cells. Methods: Stomachs, small intestines, and colons from Helicobacter felis-infected and noninfected C57BL/6 mice were examined. Double immunofluorescent staining of pSmad2/3L-Thr with Ki67, cytokeratin 8, or doublecortin and calcium/calmodulin-dependent protein kinase-like-1 (DCAMKL1) was performed, and pSmad2/3L-Thr immunostaining-positive cells were counted. After immunofluorescent staining, we stained the same sections with hematoxylin-eosin and observed these cells under a light microscope. Results: In infected mice, pSmad2/3L-Thr immunostaining-positive cells were significantly increased in the corpus and antrum compared with those of noninfected mice (p<0.0001). The number of Ki67 immunostaining-positive cells in the corpus and antrum of infected mice was also much greater than in the noninfected mice. Although pSmad2/3L-Thr immunostaining-positive cells were detected among the Ki67 cells, immunohistochemical co-localization of pSmad2/3L-Thr with Ki67 was never observed. pSmad2/3L-Thr immunostaining-positive cells showed immunohistochemical co-localization with cytokeratin 8, but some of them showed co-localization or adjacent localization with DCAMKL1 immunostaining-positive cells. Under a light microscope, pSmad2/3L-Thr immunostaining-positive cells indicated undifferentiated morphological features and were confirmed in the isthmus. In small intestines and colons, pSmad2/3L-Thr immunostaining-positive cells were detected in specific epithelial cells around crypt bases, where the respective putative stem cells are thought to exist. Conclusions: We have identified the significant expression of pSmad2/3L-Thr in specific epithelial cells of the murine stomach and have suggested these cells to be epithelial stem cells. Springer, 2010 Linker phosphorylation nationallicence Smad2/3 nationallicence Helicobacter -associated gastritis nationallicence Epithelial stem cells nationallicence Fukui Toshiro The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Kishimoto Masanobu The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Nakajima Atsushi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Yamashina Masao The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Nakayama Shinji The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Kusuda Takeo The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Sakaguchi Yutaku The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Yoshida Katsunori The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Uchida Kazushige The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Nishio Akiyoshi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Matsuzaki Koichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Okazaki Kazuichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 456-468 0944-1174 46:4<456 2011 46 535 https://doi.org/10.1007/s00535-010-0364-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0364-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukui Toshiro The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Kishimoto Masanobu The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Nakajima Atsushi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Yamashina Masao The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Nakayama Shinji The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Kusuda Takeo The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Sakaguchi Yutaku The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Yoshida Katsunori The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Uchida Kazushige The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Nishio Akiyoshi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Matsuzaki Koichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 700 1- Okazaki Kazuichi The Third Department of Internal Medicine, Division of Gastroenterology and Hepatology, Kansai Medical University, 10-15 Fumizono-cho, 570-8506, Moriguchi, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/4(2011-04-01), 456-468 0944-1174 46:4<456 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer