caa a22 4500 445888873 CHVBK 20180317145609.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00535-011-0408-8 doi (NATIONALLICENCE)springer-10.1007/s00535-011-0408-8 Differential alteration of CD56bright and CD56dim natural killer cells in frequency, phenotype, and cytokine response in chronic hepatitis C virus infection [Elektronische Daten] [Takuya Miyagi, Satoshi Shimizu, Tomohide Tatsumi, Kumiko Nishio, Naoki Hiramatsu, Tatsuya Kanto, Norio Hayashi, Tetsuo Takehara] Background: Natural killer (NK) cells play an important role in immune responses to virus infection. The cell population consists of CD56bright (bright-subset) and CD56dim (dim-subset) subsets that possess armed functions of cytokine production and cytolysis, respectively. How these subsets are involved in chronic hepatitis C virus infection (CHC) remains obscure. Methods: We investigated the frequency, phenotype, and cytokine response of these subsets in blood from CHC patients and healthy subjects (HS). Results: Dim-subset, but not bright-subset, showed lower frequency in the patients than in HS. Bright-subset from the patients more frequently expressed the NKG2A/CD94 inhibitory receptor than that from HS, while both subsets from the patients expressed lower levels of the NKG2D activating receptor. Both subsets from the patients displayed a significantly higher level of the signal transducer and activator of transcription (STAT) 1, compared with the HS. Upon stimulation with interferon-α, bright-subset activated less STAT4, required for interferon-γ production, and dim-subset activated more STAT1, required for cytolysis, in the patients than in HS. Conclusions: These results indicate alterations of NK cell subsets in frequency, phenotype, and cytokine response in CHC, which might be associated with the immune pathogenesis of CHC. Springer, 2011 NK cells nationallicence CD56bright nationallicence CD56dim nationallicence HCV nationallicence Chronic hepatitis nationallicence NK : Natural killer nationallicence IFN : Interferon nationallicence IL : Interleukin nationallicence HCV : Hepatitis C virus nationallicence CHC : Chronic hepatitis C virus infection nationallicence CHB : Chronic hepatitis B virus infection nationallicence PBMC : Peripheral blood mononuclear cell nationallicence STAT : Signal transducer and activator of transcription nationallicence pSTAT : Phosphorylated-signal transducer and activator of transcription nationallicence HS : Healthy subjects nationallicence ISG : Interferon-stimulated gene nationallicence MICA : Major histocompatibility complex class I-related chain A nationallicence Miyagi Takuya Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Shimizu Satoshi Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Tatsumi Tomohide Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Nishio Kumiko Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Hiramatsu Naoki Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Kanto Tatsuya Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Hayashi Norio Kansai-Rosai Hospital, Amagasaki, Hyogo, Japan aut Takehara Tetsuo Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut Journal of Gastroenterology Springer Japan 46/8(2011-08-01), 1020-1030 0944-1174 46:8<1020 2011 46 535 https://doi.org/10.1007/s00535-011-0408-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-011-0408-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyagi Takuya Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Shimizu Satoshi Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Tatsumi Tomohide Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Nishio Kumiko Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Hiramatsu Naoki Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Kanto Tatsuya Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 700 1- Hayashi Norio Kansai-Rosai Hospital, Amagasaki, Hyogo, Japan aut NATIONALLICENCE 700 1- Takehara Tetsuo Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/8(2011-08-01), 1020-1030 0944-1174 46:8<1020 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer